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UMC Repository: Recent submissions

  • HSF2BP Interacts with a Conserved Domain of BRCA2 and Is Required for Mouse Spermatogenesis 
    Brandsma, Inger; Sato, Koichi; van Rossum-Fikkert, Sari E.; van Vliet, Nicole; Sleddens, Esther; Reuter, Marcel; Odijk, Hanny; van den Tempel, Nathalie; Dekkers, Dick H.W.; Bezstarosti, Karel; Demmers, Jeroen A.A.; Maas, Alex; Lebbink, Joyce; Wyman, Claire; Essers, Jeroen; van Gent, Dik C.; Baarends, Willy M.; Knipscheer, Puck; Kanaar, Roland; Zelensky, Alex N. (Cell Press, 2019-06-25)
    The tumor suppressor BRCA2 is essential for homologous recombination (HR), replication fork stability, and DNA interstrand crosslink repair in vertebrates. We identify HSF2BP, a protein previously described as testis ...
  • Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids 
    Artegiani, Benedetta; van Voorthuijsen, Lisa; Lindeboom, Rik G.H.; Seinstra, Daniëlle; Heo, Inha; Tapia, Pablo; López-Iglesias, Carmen; Postrach, Daniel; Dayton, Talya; Oka, Rurika; Hu, Huili; van Boxtel, Ruben; van Es, Johan H.; Offerhaus, Johan; Peters, Peter J.; van Rheenen, Jacco; Vermeulen, Michiel; Clevers, Hans (Cell Press, 2019-06-06)
    The deubiquitinating enzyme BAP1 is a tumor suppressor, among others involved in cholangiocarcinoma. BAP1 has many proposed molecular targets, while its Drosophila homolog is known to deubiquitinate histone H2AK119. We ...
  • Absolute quantification of Cohesin, CTCF and their regulators in human cells 
    Holzmann, Johann; Politi, Antonio Z.; Nagasaka, Kota; Hantsche-Grininger, Merle; Walther, Nike; Koch, Birgit; Fuchs, Johannes; Dürnberger, Gerhard; Tang, Wen; Ladurner, Rene; Stocsits, Roman R.; Busslinger, Georg A.; Novák, Béla; Mechtler, Karl; Davidson, Iain Finley; Ellenberg, Jan; Peters, Jan Michael (eLife Sciences Publications, 2019-06-17)
    The organisation of mammalian genomes into loops and topologically associating domains (TADs) contributes to chromatin structure, gene expression and recombination. TADs and many loops are formed by cohesin and positioned ...
  • Spindle checkpoint silencing at kinetochores with submaximal microtubule occupancy 
    Etemad, Banafsheh; Vertesy, Abel; Kuijt, Timo E.F.; Sacristan, Carlos; Van Oudenaarden, Alexander; Kops, Geert J.P.L. (Company of Biologists Ltd, 2019-06-17)
    The spindle assembly checkpoint (SAC) ensures proper chromosome segregation by monitoring kinetochore-microtubule interactions. SAC proteins are shed from kinetochores once stable attachments are achieved. Human kinetochores ...
  • Creatinine synthesis rate and muscle strength and self-reported physical health in dialysis patients 
    Poppe, Enya S.J.M.; Polinder-Bos, Harmke A.; Huberts, Marleen; Vogels, Steffie; Ipema, Karin J.R.; Gansevoort, R. T.; Westerhuis, Ralf; Bakker, Stephan J.L.; Gaillard, Carlo A.J.M.; Franssen, Casper F.M. (Churchill Livingstone, 2020-05-01)
    Background & aims: Urinary creatinine excretion reflecting endogenous creatinine synthesis rate (CSR) is an established measure of muscle mass in the general populations and in patients with chronic kidney disease. There ...
  • Effect of aerobic training on vascular and metabolic risk factors for recurrent stroke: a meta-analysis 
    Brouwer, Rafaël; Wondergem, Roderick; Otten, Carlijn; Pisters, Martijn F. (Informa Healthcare, 2021-07-17)
    Aim: This meta-analysis aimed to determine the effect of aerobic training, compared to non-aerobic interventions, on vascular and metabolic risk factors for recurrent stroke. Method: This study was conducted using the ...
  • Mapping the Global Chromatin Connectivity Network for Sox2 Function in Neural Stem Cell Maintenance 
    Bertolini, Jessica A.; Favaro, Rebecca; Zhu, Yanfen; Pagin, Miriam; Ngan, Chew Yee; Wong, Chee Hong; Tjong, Harianto; Vermunt, Marit W.; Martynoga, Ben; Barone, Cristiana; Mariani, Jessica; Cardozo, Marcos Julián; Tabanera, Noemi; Zambelli, Federico; Mercurio, Sara; Ottolenghi, Sergio; Robson, Paul; Creyghton, Menno P.; Bovolenta, Paola; Pavesi, Giulio; Guillemot, Francois; Nicolis, Silvia K.; Wei, Chia Lin (Cell Press, 2019-03-07)
    Bertolini et al. report that long-range chromatin interactions in neural stem cells (NSCs) are enriched in Sox2-bound enhancers; in Sox2-deleted NSCs, interactions are reduced. Genes downregulated in Sox2-deleted cells are ...
  • Translation, cross-cultural and construct validity of the Dutch-Flemish PROMIS (R) upper extremity item bank v2.0 
    Haan, Erik Jan A.; Terwee, Caroline B.; Van Wier, Marieke F.; Willigenburg, Nienke W.; Van Deurzen, Derek F.P.; Pisters, Martijn F.; Kaat, Aaron J.; Roorda, Leo D. (Springer Netherlands, 2020-04-01)
    Purpose: To develop a Dutch–Flemish translation of the PROMIS® upper extremity (PROMIS-UE) item bank v2.0, and to investigate its cross-cultural and construct validity as well as its floor and ceiling effects in patients ...
  • Identification of Enteroendocrine Regulators by Real-Time Single-Cell Differentiation Mapping 
    Gehart, Helmuth; van Es, Johan H.; Hamer, Karien; Beumer, Joep; Kretzschmar, Kai; Dekkers, Johanna F.; Rios, Anne; Clevers, Hans (Elsevier, 2019-02-21)
    Homeostatic regulation of the intestinal enteroendocrine lineage hierarchy is a poorly understood process. We resolved transcriptional changes during enteroendocrine differentiation in real time at single-cell level using ...
  • Interactions between N-terminal Modules in MPS1 Enable Spindle Checkpoint Silencing 
    Pachis, Spyridon T.; Hiruma, Yoshitaka; Tromer, Eelco C.; Perrakis, Anastassis; Kops, Geert J.P.L. (Cell Press, 2019-02-19)
    Faithful chromosome segregation relies on the ability of the spindle assembly checkpoint (SAC) to delay anaphase onset until chromosomes are attached to the mitotic spindle via their kinetochores. MPS1 kinase is recruited ...
  • Intra-Operative Hypotension is a Risk Factor for Post-operative Silent Brain Ischaemia in Patients With Pre-operative Hypertension Undergoing Carotid Endarterectomy 
    Rots, Marjolijn L; Fassaert, Leonie M M; Kappelle, L Jaap; de Groot, Mark C H; Haitjema, Saskia; Bonati, Leo H; van Klei, Wilton A; de Borst, Gert J (W.B. Saunders Ltd, 2020-04)
    Objective: Intra-operative haemodynamic instability during carotid endarterectomy (CEA) has been associated with an increased risk of procedural stroke. Diffusion weighted imaging (DWI) lesions have been proposed as a ...
  • A ZNRF3-dependent Wnt/β-catenin signaling gradient is required for adrenal homeostasis 
    Basham, Kaitlin J.; Rodriguez, Stéphanie; Turcu, Adina F.; Lerario, Antonio M.; Logan, Catriona Y.; Rysztak, Madeline R.; Gomez-Sanchez, Celso E.; Breault, David T.; Koo, Bon Kyoung; Clevers, Hans; Nusse, Roeland; Val, Pierre; Hammer, Gary D. (Cold Spring Harbor Laboratory Press, 2019-02-01)
    Spatiotemporal control of Wnt signaling is essential for the development and homeostasis of many tissues. The transmembrane E3 ubiquitin ligases ZNRF3 (zinc and ring finger 3) and RNF43 (ring finger protein 43) antagonize ...
  • Ectopic Activation of the Spindle Assembly Checkpoint Signaling Cascade Reveals Its Biochemical Design 
    Chen, Chu; Whitney, Ian P.; Banerjee, Anand; Sacristan, Carlos; Sekhri, Palak; Kern, David M.; Fontan, Adrienne; Kops, Geert J.P.L.; Tyson, John J.; Cheeseman, Iain M.; Joglekar, Ajit P. (Cell Press, 2019-01-07)
    Switch-like activation of the spindle assembly checkpoint (SAC) is critical for accurate chromosome segregation and for cell division in a timely manner. To determine the mechanisms that achieve this, we engineered an ...
  • Tales from the crypt: new insights into intestinal stem cells 
    Gehart, Helmuth; Clevers, Hans (Nature Research, 2019-01-01)
    The intestinal epithelium withstands continuous mechanical, chemical and biological insults despite its single-layered, simple epithelial structure. The crypt–villus tissue architecture in combination with rapid cell ...
  • Organoids in immunological research 
    Bar-Ephraim, Yotam E.; Kretzschmar, Kai; Clevers, Hans (Nature Research, 2020-05-01)
    Much of our knowledge regarding the interactions between epithelial tissues and the immune system has been gathered from animal models and co-cultures with cell lines. However, unique features of human cells cannot be ...
  • Endogenous beta-galactosidase activity marks a TREM2-expressing Kupffer cell population in injured livers of Lgr5-LacZ and wild-type mice 
    Klaas, Mariliis; Mäemets-Allas, Kristina; Lõhmussaar, Kadi; Tooming, Mikk; Viil, Janeli; Jaks, Viljar (Elsevier, 2020-03)
    Lgr5-LacZ mice harbor the Escherichia coli LacZ gene encoding β-galactosidase (β-gal) under the control of the Lgr5 promoter, a stem/progenitor cell marker. In injured livers of Lgr5-LacZ mice, cells expressing β-galactosidase ...
  • Partially overlapping guidance pathways focus the activity of UNC-40/DCC along the anteroposterior axis of polarizing neuroblasts 
    Ebbing, Annabel; Middelkoop, Teije C.; Betist, Marco C.; Bodewes, Eduard; Korswagen, Hendrik C. (Company of Biologists Ltd, 2019-09-25)
    Directional migration of neurons and neuronal precursor cells is a central process in nervous system development. In the nematode Caenorhabditis elegans, the two Q neuroblasts polarize and migrate in opposite directions ...
  • Mosaic origin of the eukaryotic kinetochore 
    Tromer, Eelco C.; van Hooff, Jolien J.E.; Kops, Geert J.P.L.; Snel, Berend (National Academy of Sciences, 2019-06-25)
    The emergence of eukaryotes from ancient prokaryotic lineages embodied a remarkable increase in cellular complexity. While prokaryotes operate simple systems to connect DNA to the segregation machinery during cell division, ...
  • Glomerular function and structural integrity depend on hyaluronan synthesis by glomerular endothelium 
    Van Den Berg, Bernard M.; Wang, Gangqi; Boels, Margien G.S.; Avramut, M. Cristina; Jansen, Erik; Sol, Wendy M.P.J.; Lebrin, Franck; Van Zonneveld, Anton Jan; De Koning, Eelco J.P.; Vink, Hans; Gröne, Hermann Josef; Carmeliet, Peter; Van Der Vlag, Johan; Rabelink, Ton J. (Wolters Kluwer Health, 2019-10)
    Background A glycocalyx envelope consisting of proteoglycans and adhering proteins covers endothelial cells, both the luminal and abluminal surface.We previously demonstrated that short-term loss of integrity of the luminal ...
  • Hepatopancreatoduodenectomy –a controversial treatment for bile duct and gallbladder cancer from a European perspective 
    E-AHPBA scientific and research committee (John Wiley & Sons Inc., 2020-09)
    Background: Hepatopancreatoduodenectomy (HPD) is an aggressive operation for treatment of advanced bile duct and gallbladder cancer associated with high perioperative morbidity and mortality, and uncertain oncological ...