Abstract
Lipoxygenases are key enzymes in the metabolism of unsaturated fatty acids. Soybean lipoxygenase-1 (LOX-1), a paradigm for lipoxygenases isolated from different sources, is composed of two domains: a ~30 kDa N-terminal domain and a ~60 kDa C-terminal domain. We used limited proteolysis and gel-filtration chromatography to generate and isolate a
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