Abstract
The enzymatic synthesis of nucleotide-activated disaccharides by glycosidase-catalyzed transglycosylation reactions was investigated. High product yields were obtained when the kinetically driven syntheses were performed in frozen aqueous solutions at −5 °C. β-Galactosidase from Bacillus circulans catalyzed the transfer of D-galactose from lactose onto the nucleotide sugar UDP-Gal stereo- and regioselectively,
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