Structure of three acidic O-linked carbohydrate chains of porcine zona pellucida glycoproteins

Abstract

Structural analysis by ID and 2D 1H NMR spectroscopy of three acidic O-linked oligosaccharide alditols, released from porcine zona pellucida glycoproteins by alkaline borohydride treatment, afforded the following structures: Gal beta l-4(6SO4-)GlcNAc beta l-3Gal beta l-4GlcNAc beta 1-3Gal beta 1-3GalNAc-ol Neu5Gc alpha 2-3Gal/91 -4(6SO4-)GlcNAq beta l -3Ga1 beta l -4GlcNAc beta l -3Gal beta l -3GalNAc-ol Neu5Ac alpha 2-3Gal beta l -4(6SO4-)GlcNAc beta l -3Gal beta l -4GlcNAc beta l -3Gal beta l -3GalNAc-ol These oligosaccharides are the smallest compounds that contain the structural elements which are present in the acidic, high-molecular mass O-linked carbohydrate chains of porcine zona pellucida glycoproteins.