Abstract
Phosphorylated and nonphosphorylated tri- and tetrasaccharides were isolated from yeast mannan and analyzed by 500-MHz 1H NMR spectroscopy. Relayed spin-echo difference spectroscopy (RESED) was used to reveal subspectra of the phosphorylated residues. In this way the attachment position of the phosphate group could be established. The structures of the phosphorylated
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