α-D-Glcp-(1↔1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

Vliegenthart, J.F.G.; Fransen, C.T.M.; Laere, K.M.J. van; Wijk, A.A.C. van; Brüll, L.P.; Dignum, M.V.; Thomas-Oates, J.E.; Haverkamp, J.

doi:http://dx.doi.org/10.1016/S0008-6215(98)00285-7

α-D-Glcp-(1↔1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

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α-D-Glcp-(1↔1)-β-D-Galp-containing oligosaccharides, novel products from lactose by the action of β-galactosidase

Vliegenthart, J.F.G.; Fransen, C.T.M.; Laere, K.M.J. van; Wijk, A.A.C. van; Brüll, L.P.; Dignum, M.V.; Thomas-Oates, J.E.; Haverkamp, J.

(1998) Carbohydrate research, volume 314, issue 1-2, pp. 101 - 114

(Article)

Abstract

A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fractions obtained were analyzed using monosaccharide analysis, methylation analysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1->4)]n-alpha-D-Glcp-(1<->1)-beta-D-Galp[-(4<-1)-beta-D-Galp]m, ... read more

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DOI: https://doi.org/10.1016/S0008-6215(98)00285-7

ISSN: 0008-6215

Publisher: Elsevier

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