CiliaCarta: An integrated and validated compendium of ciliary genes
van Dam, Teunis J P; Kennedy, Julie; van der Lee, Robin; de Vrieze, Erik; Wunderlich, Kirsten A; Rix, Suzanne; Dougherty, Gerard W; Lambacher, Nils J; Li, Chunmei; Jensen, Victor L; Leroux, Michel R; Hjeij, Rim; Horn, Nicola; Texier, Yves; Wissinger, Yasmin; van Reeuwijk, Jeroen; Wheway, Gabrielle; Knapp, Barbara; Scheel, Jan F; Franco, Brunella; Mans, Dorus A; van Wijk, Erwin; Képès, François; Slaats, Gisela G; Toedt, Grischa; Kremer, Hannie; Omran, Heymut; Szymanska, Katarzyna; Koutroumpas, Konstantinos; Ueffing, Marius; Nguyen, Thanh-Minh T; Letteboer, Stef J F; Oud, Machteld M; van Beersum, Sylvia E C; Schmidts, Miriam; Beales, Philip L; Lu, Qianhao; Giles, Rachel H; Szklarczyk, Radek; Russell, Robert B; Gibson, Toby J; Johnson, Colin A; Blacque, Oliver E; Wolfrum, Uwe; Boldt, Karsten; Roepman, Ronald; Hernandez-Hernandez, Victor; Huynen, Martijn A
(2019) PLoS ONE, volume 14, issue 5
(Article)
Abstract
The cilium is an essential organelle at the surface of mammalian cells whose dysfunction causes a wide range of genetic diseases collectively called ciliopathies. The current rate at which new ciliopathy genes are identified suggests that many ciliary components remain undiscovered. We generated and rigorously analyzed genomic, proteomic, transcriptomic and
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evolutionary data and systematically integrated these using Bayesian statistics into a predictive score for ciliary function. This resulted in 285 candidate ciliary genes. We generated independent experimental evidence of ciliary associations for 24 out of 36 analyzed candidate proteins using multiple cell and animal model systems (mouse, zebrafish and nematode) and techniques. For example, we show that OSCP1, which has previously been implicated in two distinct non-ciliary processes, causes ciliogenic and ciliopathy-associated tissue phenotypes when depleted in zebrafish. The candidate list forms the basis of CiliaCarta, a comprehensive ciliary compendium covering 956 genes. The resource can be used to objectively prioritize candidate genes in whole exome or genome sequencing of ciliopathy patients and can be accessed at http://bioinformatics.bio.uu.nl/john/syscilia/ciliacarta/.
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Keywords: Animals, Bayes Theorem, Caenorhabditis elegans/cytology, Cilia/genetics, Genomics, Molecular Sequence Annotation, Phenotype, Reproducibility of Results, Sensory Receptor Cells/metabolism, Zebrafish/genetics, Journal Article, Research Support, Non-U.S. Gov't
ISSN: 1932-6203
Publisher: Public Library of Science
Note: Publisher Copyright: © 2019 van Dam et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
(Peer reviewed)