Human microglial models to study HIV infection and neuropathogenesis: a literature overview and comparative analyses

Gumbs, Stephanie B.H.; Kübler, Raphael; Gharu, Lavina; Schipper, Pauline J.; Borst, Anne L.; Snijders, Gijsje J.L.J.; Ormel, Paul R.; van Berlekom, Amber Berdenis; Wensing, Annemarie M.J.; de Witte, Lot D.; Nijhuis, Monique

doi:https://doi.org/10.1007/s13365-021-01049-w

Human microglial models to study HIV infection and neuropathogenesis: a literature overview and comparative analyses

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Human microglial models to study HIV infection and neuropathogenesis: a literature overview and comparative analyses

Gumbs, Stephanie B.H.; Kübler, Raphael; Gharu, Lavina; Schipper, Pauline J.; Borst, Anne L.; Snijders, Gijsje J.L.J.; Ormel, Paul R.; van Berlekom, Amber Berdenis; Wensing, Annemarie M.J.; de Witte, Lot D.; Nijhuis, Monique

(2022) Journal of Neurovirology, volume 28, issue 1, pp. 64 - 91

(Article)

Abstract

HIV persistence in the CNS despite antiretroviral therapy may cause neurological disorders and poses a critical challenge for HIV cure. Understanding the pathobiology of HIV-infected microglia, the main viral CNS reservoir, is imperative. Here, we provide a comprehensive comparison of human microglial culture models: cultured primary microglia (pMG), microglial cell ... read more

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Keywords: Central nervous system, HIV, HIV-associated neurocognitive disorder, Microglia, Neuropathogenesis, Organoid, Humans, Cells, Cultured, Microglia/pathology, Monocytes, Virus Replication, HIV-1/genetics, HIV Infections/pathology, Clinical Neurology, Neurology, Cellular and Molecular Neuroscience, Virology, Review, Research Support, Non-U.S. Gov't, Journal Article, Research Support, N.I.H., Extramural

DOI: https://doi.org/10.1007/s13365-021-01049-w

ISSN: 1355-0284

Publisher: Springer New York

Note: Funding Information: This work was financially supported by grants from the Aidsfonds (P‐13204; P‐37203, Health Holland (LSHM2OO12‐SGF) and NIHM (R21MH120581). The authors thank the team of the Netherlands Brain Bank ( http://www.brainbank.nl ) for their services. We thank the study participants for their generous gift of brain donation. We are grateful to The MIND Facility and Daniëlle Vonk, who helped with fibroblasts and iPSC culture and characterization, and to F.A.J. Gigase for teaching and advice on organoid differentiation and culture. Funding Information: This work was financially supported by grants from the Aidsfonds (P‐13204; P‐37203, Health Holland (LSHM2OO12‐SGF) and NIHM (R21MH120581). The authors thank the team of the Netherlands Brain Bank (http://www.brainbank.nl) for their services. We thank the study participants for their generous gift of brain donation. We are grateful to The MIND Facility and Daniëlle Vonk, who helped with fibroblasts and iPSC culture and characterization, and to F.A.J. Gigase for teaching and advice on organoid differentiation and culture. Publisher Copyright: © 2022, The Author(s). © 2022. The Author(s).

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