Fecal amine metabolite analysis before onset of severe necrotizing enterocolitis in preterm infants: a prospective case-control study
Deianova, N; El Manouni El Hassani, S; Struijs, E A; Jansen, E E W; Bakkali, A; van de Wiel, M A; de Boode, Willem P; Hulzebos, C V; van Kaam, A H; Kramer, B W; d'Haens, E; Vijlbrief, D C; van Weissenbruch, M M; de Jonge, W J; Benninga, M A; Niemarkt, H J; de Boer, N K H; de Meij, T G J
(2022) Scientific Reports, volume 12, issue 1
(Article)
Abstract
Infants developing necrotizing enterocolitis (NEC) have a different metabolomic profile compared to controls. The potential of specific metabolomics, i.e. amino acids and amino alcohols (AAA), as early diagnostic biomarkers for NEC is largely unexplored. In this multicenter prospective case-control study, longitudinally collected fecal samples from preterm infants (born <30 weeks
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of gestation) from 1-3 days before diagnosis of severe NEC (Bell's stage IIIA/IIIB), were analyzed by targeted high-performance liquid chromatography (HPLC). Control samples were collected from gestational and postnatal age-matched infants. Thirty-one NEC cases (15 NEC IIIA;16 NEC IIIB) with 1:1 matched controls were included. Preclinical samples of infants with NEC were characterized by five increased essential amino acids-isoleucine, leucine, methionine, phenylalanine and valine. Lysine and ethanolamine ratios were lower prior to NEC, compared to control samples. A multivariate model was rendered based on isoleucine, lysine, ethanolamine, tryptophan and ornithine, modestly discriminating cases from controls (AUC 0.67; p < 0.001). Targeted HPLC pointed to several specific AAA alterations in samples collected 1-3 days before NEC onset, compared to controls. Whether this reflects metabolic alterations and has a role in early biomarker development for NEC, has yet to be elucidated.
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Keywords: Amines, Case-Control Studies, Enterocolitis, Necrotizing/diagnosis, Ethanolamines, Humans, Infant, Infant, Newborn, Infant, Newborn, Diseases, Infant, Premature/metabolism, Isoleucine, Lysine, Journal Article, Multicenter Study
ISSN: 2045-2322
Publisher: Nature Publishing Group
Note: Funding Information: This work was supported by the European Union’s Horizon 2020 research and innovation program under the MarieSkłodowska-Curie grant agreement [Grant number 814168], as well as the MaagLeverDarmStichting,Netherlands grant agreement [Grant number WOO 19-14]. The funding sources had no role in the design and conduct of the study. Publisher Copyright: © 2022, The Author(s).
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