Mechanistic basis for Sgo1-mediated centromere localization and function of the CPC
Abad, Maria Alba; Gupta, Tanmay; Hadders, Michael A; Meppelink, Amanda; Wopken, J Pepijn; Blackburn, Elizabeth; Zou, Juan; Gireesh, Anjitha; Buzuk, Lana; Kelly, David A; McHugh, Toni; Rappsilber, Juri; Lens, Susanne M A; Jeyaprakash, A Arockia
(2022) The Journal of cell biology, volume 221, issue 8, pp. 1 - 22
(Article)
Abstract
Centromere association of the chromosomal passenger complex (CPC; Borealin-Survivin-INCENP-Aurora B) and Sgo1 is crucial for chromosome biorientation, a process essential for error-free chromosome segregation. Phosphorylated histone H3 Thr3 (H3T3ph; directly recognized by Survivin) and histone H2A Thr120 (H2AT120ph; indirectly recognized via Sgo1), together with CPC's intrinsic nucleosome-binding ability, facilitate CPC
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centromere recruitment. However, the molecular basis for CPC-Sgo1 binding and how their physical interaction influences CPC centromere localization are lacking. Here, using an integrative structure-function approach, we show that the "histone H3-like" Sgo1 N-terminal tail-Survivin BIR domain interaction acts as a hotspot essential for CPC-Sgo1 assembly, while downstream Sgo1 residues and Borealin contribute for high-affinity binding. Disrupting Sgo1-Survivin interaction abolished CPC-Sgo1 assembly and perturbed CPC centromere localization and function. Our findings reveal that Sgo1 and H3T3ph use the same surface on Survivin to bind CPC. Hence, it is likely that these interactions take place in a spatiotemporally restricted manner, providing a rationale for the Sgo1-mediated "kinetochore-proximal" CPC centromere pool.
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Keywords: Cell Cycle Proteins/genetics, Centromere/metabolism, Histones/genetics, Kinetochores/metabolism, Phosphorylation, Survivin/genetics, Cell Biology, Journal Article
ISSN: 0021-9525
Publisher: Rockefeller University Press
Note: Funding Information: We thank the staff of the Edinburgh Protein Production Facility and the Centre for Optical Instrumentation Laboratory for their help. We also thank Ana Losada (Chromosome Dynamics Group, Molecular Oncology Programme, Spanish National Cancer Research Centre, Madrid, Spain) for kindly providing the Sgo1 antibody and William C. Earnshaw (Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, UK) for kindly providing the CENP-C antibody. We thank Refeyn Ltd. (Oxford, UK), especially Akhila Bettadapur and Tomás de Garay, for their help with the mass photometry experiments. We thank Cristina Ferrás and Marco Cruz (Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal) for sharing with us the Sgo1 siRNA oligonucleotide sequence. We thank Bethan Medina-Pritchard for critical reading of the manuscript. The Wellcome Trust generously supported this work through a Career Development and Enhancement Grant (095822) and Senior Research Fellowship (202811) to A.A. Jeyaprakash and a Centre Core Grant (203149) and a Core Grant (109916/Z/15/Z) to the Edinburgh Protein Production Facility. This study was also supported by a research grant from the Dutch Cancer Society (KWF grant 10366) to S.M.A. Lens. Moreover, the Lens lab is part of Oncode Institute, which is partly financed by the Dutch Cancer Society. Tanmay Gupta was funded by the Darwin Trust of Edinburgh. The authors declare no competing financial interests. Funding Information: The Wellcome Trust generously supported this work through a Career Development and Enhancement Grant (095822) and Senior Research Fellowship (202811) to A.A. Jeyaprakash and a Centre Core Grant (203149) and a Core Grant (109916/Z/15/Z) to the Edinburgh Protein Production Facility. This study was also supported by a research grant from the Dutch Cancer Society (KWF grant 10366) to S.M.A. Lens. Moreover, the Lens lab is part of On-code Institute, which is partly financed by the Dutch Cancer Society. Tanmay Gupta was funded by the Darwin Trust of Edinburgh. The authors declare no competing financial interests. Publisher Copyright: © 2022 Abad et al.
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