Transurethral versus suprapubic catheterization to test urethral function in rats
Janssen, Kristine; Deng, Kangli; Majerus, Steve J.A.; Lin, Dan Li; Hanzlicek, Brett; Butler, Robert S.; van der Vaart, Carl H.; Damaser, Margot S.
(2021) Scientific Reports, volume 11, issue 1, pp. 1 - 8
(Article)
Abstract
Transurethral and suprapubic catheterization have both been used to test urethral function in rats; however, it is unknown whether these methods affect urethral function or if the order of catheterization affects the results. The aim of this cross-over designed experiment was to compare the effects of catheterization methods and order
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on leak point pressure (LPP) testing. LPP and simultaneous external urethral sphincter electromyography (EUS EMG) were recorded in anesthetized female virgin Sprague-Dawley rats in a cross-over design to test the effects of transurethral and suprapubic catheterization. There was no significant difference in peak bladder pressure during LPP testing whether measured with a transurethral or suprapubic catheter. There was no significant difference in peak bladder pressure between the first and second catheter insertions. However, peak EMG firing rate, as well as peak EMG amplitude and EMG amplitude difference between peak and baseline were significantly higher after the first catheter insertion compared to the second insertion, regardless of the catheter method. Our results suggest that route of catheterization does not alter urethral function, e.g. create a functional partial outlet obstruction. Either catheterization method could be used for LPP and/or EUS EMG testing in rats.
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Keywords: Animals, Electromyography, Female, Pressure, Rats, Rats, Sprague-Dawley, Reproducibility of Results, Urethra/physiology, Urinary Bladder/physiology, Urinary Catheterization/methods, Urination, Urodynamics, Urology/instrumentation, General, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Journal Article, Comparative Study
ISSN: 2045-2322
Publisher: Nature Publishing Group
Note: Funding Information: This work was supported by the Rehabilitation Research & Development Service of the Department of Veterans Affairs Merit Review Award I01 RX000228 and the Cleveland Clinic. Publisher Copyright: © 2021, The Author(s).
(Peer reviewed)