The frontier of live tissue imaging across space and time
Huang, Qiang; Garrett, Aliesha; Bose, Shree; Blocker, Stephanie; Rios, Anne C; Clevers, Hans; Shen, Xiling
(2021) Cell stem cell, volume 28, issue 4, pp. 603 - 622
(Article)
Abstract
What you see is what you get-imaging techniques have long been essential for visualization and understanding of tissue development, homeostasis, and regeneration, which are driven by stem cell self-renewal and differentiation. Advances in molecular and tissue modeling techniques in the last decade are providing new imaging modalities to explore tissue
... read more
heterogeneity and plasticity. Here we describe current state-of-the-art imaging modalities for tissue research at multiple scales, with a focus on explaining key tradeoffs such as spatial resolution, penetration depth, capture time/frequency, and moieties. We explore emerging tissue modeling and molecular tools that improve resolution, specificity, and throughput.
show less
Download/Full Text
The full text of this publication is not available.
Keywords: Molecular Medicine, Genetics, Cell Biology
ISSN: 1934-5909
Publisher: Cell Press
Note: Funding Information: We thank Dr. Michael Hancock for his review and comments. We thank Dr. Gabriele Bixel at the Max Planck Institute for Molecular Biomedicine (Münster, Germany), Dr. Max Nobis and Dr. Paul Timpson at the Garvan Institute of Medical Research (Darlinghurst, Australia), Haohua Tu and Stephen Boppart at the University of Illinois Biophotonics Imaging Laboratory (Urbana, IL), Al Johnson at the Duke University Center for In Vivo Microscopy (Durham, NC), and Junjie Yao at Duke University (Durham, NC) for providing previously unpublished images for our figures. The work was supported by NIH R35GM122465 (to X.S.), DK119795 (to X.S), NSFC81670468 (to Q.H), 2017KJXX-43 (to Q.H), and 2018SF-208 (to Q.H). The authors declare no competing interests. Funding Information: We thank Dr. Michael Hancock for his review and comments. We thank Dr. Gabriele Bixel at the Max Planck Institute for Molecular Biomedicine (Münster, Germany), Dr. Max Nobis and Dr. Paul Timpson at the Garvan Institute of Medical Research (Darlinghurst, Australia), Haohua Tu and Stephen Boppart at the University of Illinois Biophotonics Imaging Laboratory (Urbana, IL), Al Johnson at the Duke University Center for In Vivo Microscopy (Durham, NC), and Junjie Yao at Duke University (Durham, NC) for providing previously unpublished images for our figures. The work was supported by NIH R35GM122465 (to X.S.), DK119795 (to X.S), NSFC81670468 (to Q.H), 2017KJXX-43 (to Q.H), and 2018SF-208 (to Q.H). Publisher Copyright: © 2021 Elsevier Inc.
(Peer reviewed)