A Plasma Creatinine- and Urea-Based Equation to Estimate Glomerular Filtration Rate in Rats
Besseling, Paul J; Pieters, Tobias Theodorus; Nguyen, Isabel T N; de Bree, Petra M; Willekes, Nel; Dijk, Adele H; Bovée, Dominique M; Hoorn, Ewout J; Rookmaaker, Maarten B; Gerritsen, Karin G; Verhaar, Marianne C; Gremmels, Hendrik; Joles, Jaap A
(2021) American journal of physiology. Renal physiology, volume 320, issue 3, pp. F518 - F524
(Article)
Abstract
Monitoring renal function is a vital part of kidney research involving rats. The laborious measurement of glomerular filtration rate (GFR) with administration of exogenous filtration markers does not easily allow serial measurements. Using an in-house database of inulin clearances, we developed and validated a plasma creatinine- and plasma urea-based equation
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to estimate GFR in a large cohort of male rats [development cohort n = 325, R 2 = 0.816, percentage of predictions that fell within 30% of the true value (P30) = 76%] that had high accuracy in the validation cohort ( n = 116 rats, R 2 = 0.935, P30 = 79%). The equation was less accurate in rats with nonsteady-state creatinine, in which the equation should therefore not be used. In conclusion, applying this equation facilitates easy and repeatable estimates of GFR in rats. NEW & NOTEWORTHY This is the first equation, that we know of, which estimates glomerular filtration rate in rats based on a single measurement of body weight, plasma creatinine, and plasma urea.
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Keywords: Creatinine, Estimated glomerular filtration rate, Inulin clearance, Rats, Urea, Urology, Physiology, Journal Article
ISSN: 1931-857X
Publisher: American Physiological Society
Note: Funding Information: This work was supported by The Netherlands CardioVascular Research Initiative: an initiative with support of the Dutch Heart Foundation [Grant No. CVON2014-11 (RECONNECT)], by ZonMw within the LSH 2Treat programme and the Dutch Kidney Foundation [Grant No. 436001003 (InSiTeVx)], and by the Alexandre Suerman Stipend from UMC Utrecht. Publisher Copyright: © 2021 the American Physiological Society
(Peer reviewed)