Cellulose Nanofibril Hydrogel Promotes Hepatic Differentiation of Human Liver Organoids
Krüger, Melanie; Oosterhoff, Loes A; van Wolferen, Monique E; Schiele, Simon A; Walther, Andreas; Geijsen, Niels; De Laporte, Laura; van der Laan, Luc J W; Kock, Linda M; Spee, Bart
(2020) Advanced Healthcare Materials, volume 9, issue 6, pp. 1 - 12
(Article)
Abstract
To replicate functional liver tissue in vitro for drug testing or transplantation, 3D tissue engineering requires representative cell models as well as scaffolds that not only promote tissue production but also are applicable in a clinical setting. Recently, adult liver-derived liver organoids are found to be of much interest due
... read more
to their genetic stability, expansion potential, and ability to differentiate toward a hepatocyte-like fate. The current standard for culturing these organoids is a basement membrane hydrogel like Matrigel (MG), which is derived from murine tumor material and apart from its variability and high costs, possesses an undefined composition and is therefore not clinically applicable. Here, a cellulose nanofibril (CNF) hydrogel is investigated with regard to its potential to serve as an alternative clinical grade scaffold to differentiate liver organoids. The results show that its mechanical properties are suitable for differentiation with overall, either equal or improved, functionality of the hepatocyte-like cells compared to MG. Therefore, and because of its defined and tunable chemical definition, the CNF hydrogel presents a viable alternative to MG for liver tissue engineering with the option for clinical use.
show less
Download/Full Text
Keywords: cellulose nanofibril hydrogels, clinical-grade scaffolds, engineered tissues, liver organoid scaffolds, liver organoids, Matrigel, Biomedical Engineering, Biomaterials, Pharmaceutical Science, Research Support, Non-U.S. Gov't, Journal Article
ISSN: 2192-2640
Publisher: John Wiley and Sons Ltd
Note: Funding Information: This work received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 64268 and was supported by a grant from the Dutch Research Council NWO STW (15498) to B.S. The authors thank the Veterinary Pathology Diagnostics Centre, Utrecht, for performing the PAS staining and Dr. Monique Verstegen from the Erasmus Medical Center Rotterdam for liver organoid initiation. Funding Information: This work received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska‐Curie grant agreement No 64268 and was supported by a grant from the Dutch Research Council NWO STW (15498) to B.S. The authors thank the Veterinary Pathology Diagnostics Centre, Utrecht, for performing the PAS staining and Dr. Monique Verstegen from the Erasmus Medical Center Rotterdam for liver organoid initiation. Publisher Copyright: © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
(Peer reviewed)