GlioM&M: Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma
Abels, Erik R.; Maas, Sybren L.N.; Tai, Eric; Ting, David T.; Broekman, Marike L.D.; Breakefield, Xandra O.; El Khoury, Joseph
(2020) Scientific Reports, volume 10, issue 1, pp. 1 - 11
(Article)
Abstract
Monocytes, macrophages and microglia make up a large part of the glioma environment and have an important role in maintaining and propagating glioma progression. Targeting these cells to inhibit their tumor-promoting effect and reprogramming them into an anti-tumor phenotype is a potential therapeutic approach for glioma. In this study we
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analyzed the transcriptomes of eight different monocyte subgroups derived from the brain and the blood of glioma-bearing mice. We compared the expression profile of blood-derived monocytes versus tumor-infiltrating monocytes and found increased expression of both pro- and anti-inflammatory pathways in tumor infiltrating monocytes. To help disseminate these datasets, we created a user-friendly web-based tool accessible at www.glioma-monocytes.com. This tool can be used for validation purposes and to elucidate gene expression profiles of tumor-interacting monocytes and macrophages as well as blood-derived circulating monocytes. This tool can also be used to identify new markers and targets for therapy in these different cell populations.
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Keywords: Animals, Biomarkers/metabolism, Brain Neoplasms/immunology, Cell Line, Tumor, Cytokines/metabolism, Databases, Factual, Disease Models, Animal, Glioma/immunology, Lymphocytes, Tumor-Infiltrating/cytology, Macrophages/cytology, Mice, Mice, Inbred C57BL, Monocytes/cytology, Transplantation, Homologous, Up-Regulation, User-Computer Interface, Journal Article, Research Support, N.I.H., Extramural
ISSN: 2045-2322
Publisher: Nature Publishing Group
Note: Funding Information: We thank the Massachusetts General Hospital, Department of Pathology Flow and Image Cytometry Research Core. All members of the Breakefield, Tannous, Maguire, Bragg and El Khoury labs that suggested ideas during lab meetings are very much appreciated. We thank Ms. Suzanne McDavitt for skilled editorial assistance. Sybren Maas acknowledges support from the Dutch Nijbakker-Morra travel stipend and the Dutch Cancer Society (KWF) travel grant. Xandra Breakefield acknowledges NIH NCI CA179563, CA069246 and CA232103 grants for funding used to perform this research. U19 CA179563 is supported by the NIH Common Fund, through the Office of Strategic Coordination/Office of the NIH Director. Joseph El Khoury is funded by NIH grants 1RF1 AG051506 and R01 AI119065. Generation of vectors used in this study was supported by the NIH NS045776 grant. The MGH Department of Pathology Flow and Image Cytometry Research Core obtained support from the NIH Shared Instrumentation program with grants 1S10OD012027-01A1, 1S10OD016372-01, 1S10RR020936-01, and 1S10RR023440-01A1. Publisher Copyright: © 2020, The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
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