Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes

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Wnt Activation and Reduced Cell-Cell Contact Synergistically Induce Massive Expansion of Functional Human iPSC-Derived Cardiomyocytes
 
Buikema, Jan W; Lee, Soah; Goodyer, William R; Maas, Renee G; Chirikian, Orlando; Li, Guang; Miao, Yi; Paige, Sharon L; Lee, Daniel; Wu, Haodi; Paik, David T; Rhee, Siyeon; Tian, Lei; Galdos, Francisco X; Puluca, Nazan; Beyersdorf, Benjamin; Hu, James; Beck, Aimee; Venkamatran, Sneha; Swami, Srilatha; Wijnker, Paul; Schuldt, Maike; Dorsch, Larissa M; van Mil, Alain; Red-Horse, Kristy; Wu, Joy Y; Geisen, Caroline; Hesse, Michael; Serpooshan, Vahid; Jovinge, Stefan; Fleischmann, Bernd K; Doevendans, Pieter A; van der Velden, Jolanda; Garcia, K Christopher; Wu, Joseph C; Sluijter, Joost P G; Wu, Sean M
(2020) Cell stem cell, volume 27, issue 1, pp. 50 - 63.e5
(Article)
Abstract
Deriving a large number of hiPSC-cardiomyocytes would be beneficial for large-scale tissue engineering and drug screening applications. Buikema et al. show that GSK-3β inhibition combined with removal of cell-cell contact enables massive expansion of hiPSC-cardiomyocytes with comparable function to non-expanded cells.
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Keywords: GSK3, Wnt signaling, cardiomyocytes, expansion, induced pluripotent stem cells, maturation, proliferation, Journal Article
DOI: https://doi.org/10.1016/j.stem.2020.06.001
ISSN: 1934-5909
Publisher: Cell Press
Note: Publisher Copyright: © 2020 Elsevier Inc.
(Peer reviewed)