SARS-CoV-2 productively infects human gut enterocytes
Lamers, Mart M; Beumer, Joep; van der Vaart, Jelte; Knoops, Kèvin; Puschhof, Jens; Breugem, Tim I; Ravelli, Raimond B G; Paul van Schayck, J; Mykytyn, Anna Z; Duimel, Hans Q; van Donselaar, Elly; Riesebosch, Samra; Kuijpers, Helma J H; Schippers, Debby; van de Wetering, Willine J; de Graaf, Miranda; Koopmans, Marion; Cuppen, Edwin; Peters, Peter J; Haagmans, Bart L; Clevers, Hans
(2020) Science, volume 369, issue 6499, pp. 50 - 54
(Article)
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can cause coronavirus disease 2019 (COVID-19), an influenza-like disease that is primarily thought to infect the lungs with transmission through the respiratory route. However, clinical evidence suggests that the intestine may present another viral target organ. Indeed, the SARS-CoV-2 receptor angiotensin-converting enzyme 2
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(ACE2) is highly expressed on differentiated enterocytes. In human small intestinal organoids (hSIOs), enterocytes were readily infected by SARS-CoV and SARS-CoV-2, as demonstrated by confocal and electron microscopy. Enterocytes produced infectious viral particles, whereas messenger RNA expression analysis of hSIOs revealed induction of a generic viral response program. Therefore, the intestinal epithelium supports SARS-CoV-2 replication, and hSIOs serve as an experimental model for coronavirus infection and biology.
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Keywords: Betacoronavirus/physiology, Cell Culture Techniques, Cell Differentiation, Cell Lineage, Cell Proliferation, Culture Media, Enterocytes/metabolism, Gene Expression, Humans, Ileum/metabolism, Lung/virology, Male, Organoids, Peptidyl-Dipeptidase A/genetics, RNA, Messenger/genetics, Receptors, Virus/genetics, Respiratory Mucosa/virology, SARS Virus/physiology, Virus Replication, General, Journal Article, Research Support, Non-U.S. Gov't
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science
Note: Publisher Copyright: © 2020 American Association for the Advancement of Science. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
(Peer reviewed)
