Complement factor H and apolipoprotein E participate in regulation of inflammation in THP-1 macrophages
Nissilä, Eija; Hakala, Pipsa; Leskinen, Katarzyna; Roig, Angela; Syed, Shahan; Van Kessel, Kok P.M.; Metso, Jari; De Haas, Carla J.C.; Saavalainen, Päivi; Meri, Seppo; Chroni, Angeliki; Van Strijp, Jos A.G.; Öörni, Katariina; Jauhiainen, Matti; Jokiranta, T. Sakari; Haapasalo, Karita
(2018) Frontiers in Immunology, volume 9, issue NOV
(Article)
Abstract
The alternative pathway (AP) of complement is constantly active in plasma and can easily be activated on self surfaces and trigger local inflammation. Host cells are protected from AP attack by Factor H (FH), the main AP regulator in plasma. Although complement is known to play a role in atherosclerosis,
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the mechanisms of its contribution are not fully understood. Since FH via its domains 5-7 binds apoliporotein E (apoE) and macrophages produce apoE we examined how FH could be involved in the antiatherogenic effects of apoE. We used blood peripheral monocytes and THP-1 monocyte/macrophage cells which were also loaded with acetylated low-density lipoprotein (LDL) to form foam cells. Binding of FH and apoE on these cells was analyzed by flow cytometry. High-density lipoprotein (HDL)-mediated cholesterol efflux of activated THP-1 cells was measured and transcriptomes of THP-1 cells using mRNA sequencing were determined. We found that binding of FH to human blood monocytes and cholesterol-loaded THP-1 macrophages increased apoE binding to these cells. Preincubation of fluorescent cholesterol labeled THP-1 macrophages in the presence of FH increased cholesterol efflux and cholesterol-loaded macrophages displayed reduced transcription of proinflammatory/proatherogenic factors and increased transcription of anti-inflammatory/anti-atherogenic factors. Further incubation of THP-1 cells with serum reduced C3b/iC3b deposition. Overall, our data indicate that apoE and FH interact with monocytic cells in a concerted action and this interaction reduces complement activation and inflammation in the atherosclerotic lesions. By this way FH may participate in mediating the beneficial effects of apoE in suppressing atherosclerotic lesion progression.
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Keywords: Apolipoprotein E, Atherosclerosis, Complement, Complement system, Factor H, Inflammation, Immunology and Allergy, Immunology, Research Support, Non-U.S. Gov't, Journal Article
ISSN: 1664-3224
Publisher: Frontiers Media S. A.
Note: Funding Information: The work was supported by The Finnish Cultural Foundation (KH), Jane and Aatos Erkko Foundation (to MJ and JM) and Sigrid Juselius Foundation. Publisher Copyright: © 2007 - 2018 Frontiers Media S.A. All Rights Reserved.
(Peer reviewed)