Monolithic liquid-chromatography columns for protein analysisprotein digest separation and integrated systems

Abstract

The objectives of the research described in this thesis are to evaluate the applicability of both silica-based and polymeric monolithic columns for protein analysis. The first part describes investigations into the effects of column length and stationary-phase chemistry on the separation of protein digests. The second part is devoted to the development of LC based integrated systems for the separation and identification of protein mixtures.