Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

DSpace/Manakin Repository

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition
 
Timmers, Matt; Peeters, Wouter; Hauwert, Niels J; Rijcken, Cristianne J F; Vermonden, Tina; Dijkgraaf, Ingrid; Liskamp, Rob M J
(2023) Chemical communications (Cambridge, England), volume 59, issue 76, pp. 11397 - 11400
(Article)
Abstract
The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.
Download/Full Text
Open Access version via Utrecht University Repository Publisher version
 
Keywords: Azides, Cycloaddition Reaction, Peptides, Antibodies, Alkynes, Electronic, Optical and Magnetic Materials, Ceramics and Composites, Metals and Alloys, Materials Chemistry, Surfaces, Coatings and Films, General Chemistry, Catalysis
DOI: https://doi.org/10.1039/d3cc03397j
ISSN: 1359-7345
Publisher: Royal Society of Chemistry
Note: Publisher Copyright: © 2023 The Royal Society of Chemistry.
(Peer reviewed)
 
 
See more statistics about this item