The conserved iol gene cluster in Pseudomonas is involved in rhizosphere competence
Sánchez-Gil, Juan J; Poppeliers, Sanne W M; Vacheron, Jordan; Zhang, Hao; Odijk, Bart; Keel, Christoph; de Jonge, Ronnie
(2023) Current Biology, volume 33, issue 15, pp. 3097 - 3110.e6
(Article)
Abstract
The Pseudomonas genus has shown great potential as a sustainable solution to support agriculture through its plant-growth-promoting and biocontrol activities. However, their efficacy as bioinoculants is limited by unpredictable colonization in natural conditions. Our study identifies the iol locus, a gene cluster in Pseudomonas involved in inositol catabolism, as a
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feature enriched among superior root colonizers in natural soil. Further characterization revealed that the iol locus increases competitiveness, potentially caused by an observed induction of swimming motility and the production of fluorescent siderophore in response to inositol, a plant-derived compound. Public data analyses indicate that the iol locus is broadly conserved in the Pseudomonas genus and linked to diverse host-microbe interactions. Together, our findings suggest the iol locus as a potential target for developing more effective bioinoculants for sustainable agriculture.
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Keywords: Pseudomonas/genetics, Rhizosphere, Agriculture, Soil Microbiology, Plant Development, Plant Roots/genetics, Taverne
ISSN: 0960-9822
Publisher: Cell Press
Note: Funding Information: The authors want to thank Bas E. Dutilh, Corné M.J. Pieterse, and members of the Plant-Microbe Interactions lab for helpful discussions. We acknowledge the Utrecht Sequencing Facility (USEQ) for providing sequencing service and data. USEQ is subsidized by the University Medical Center Utrecht and the Netherlands X-omics Initiative ( NWO project 184.034.019 ). This study was supported by the NWO Green II grant no. ALWGR.2017.002 (S.W.M.P., J.J.S.-G., and R.d.J.), the Novo Nordisk Foundation grant no. NNF19SA0059362 (R.d.J.), and the China Scholarship Council scholarship no. 201406300090 (H.Z.). Funding Information: The authors want to thank Bas E. Dutilh, Corné M.J. Pieterse, and members of the Plant-Microbe Interactions lab for helpful discussions. We acknowledge the Utrecht Sequencing Facility (USEQ) for providing sequencing service and data. USEQ is subsidized by the University Medical Center Utrecht and the Netherlands X-omics Initiative (NWO project 184.034.019). This study was supported by the NWO Green II grant no. ALWGR.2017.002 (S.W.M.P. J.J.S.-G. and R.d.J.), the Novo Nordisk Foundation grant no. NNF19SA0059362 (R.d.J.), and the China Scholarship Council scholarship no. 201406300090 (H.Z.). Conceptualization, J.J.S.-G.; methodology, J.J.S.-G. J.V. C.K. and H.Z.; software, J.J.S.-G.; validation, J.J.S.-G. and R.d.J.; formal analysis, J.J.S.-G. and R.d.J.; investigation, J.J.S.-G. S.W.M.P. and B.O.; resources, J.V. C.K. and H.Z.; data curation, J.J.S.-G.; writing – original draft, J.J.S.-G.; writing – review & editing, J.V. C.K. and R.d.J.; supervision, R.d.J.; project administration, R.d.J.; funding acquisition, R.d.J. The authors declare no competing interests. Publisher Copyright: © 2023 Elsevier Inc.
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