Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms
Yang, Yuankun; Steidele, Christina E; Rössner, Clemens; Löffelhardt, Birgit; Kolb, Dagmar; Leisen, Thomas; Zhang, Weiguo; Ludwig, Christina; Felix, Georg; Seidl, Michael F; Becker, Annette; Nürnberger, Thorsten; Hahn, Matthias; Gust, Bertolt; Gross, Harald; Hückelhoven, Ralph; Gust, Andrea A
(2023) Nature Communications, volume 14, issue 1, pp. 1 - 12
(Article)
Abstract
The Arabidopsis thaliana Receptor-Like Protein RLP30 contributes to immunity against the fungal pathogen Sclerotinia sclerotiorum. Here we identify the RLP30-ligand as a small cysteine-rich protein (SCP) that occurs in many fungi and oomycetes and is also recognized by the Nicotiana benthamiana RLP RE02. However, RLP30 and RE02 share little sequence
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similarity and respond to different parts of the native/folded protein. Moreover, some Brassicaceae other than Arabidopsis also respond to a linear SCP peptide instead of the folded protein, suggesting that SCP is an eminent immune target that led to the convergent evolution of distinct immune receptors in plants. Surprisingly, RLP30 shows a second ligand specificity for a SCP-nonhomologous protein secreted by bacterial Pseudomonads. RLP30 expression in N. tabacum results in quantitatively lower susceptibility to bacterial, fungal and oomycete pathogens, thus demonstrating that detection of immunogenic patterns by Arabidopsis RLP30 is involved in defense against pathogens from three microbial kingdoms.
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Keywords: Arabidopsis/metabolism, Cysteine/metabolism, Ligands, Proteins/metabolism, Oomycetes/metabolism, Bacteria/metabolism, Receptors, Pattern Recognition/metabolism, Plant Diseases/microbiology, Plant Immunity, Arabidopsis Proteins/genetics, Gene Expression Regulation, Plant, General Physics and Astronomy, General Chemistry, General Biochemistry,Genetics and Molecular Biology
ISSN: 2041-1723
Publisher: Nature Publishing Group
Note: Funding Information: We thank M. H. A. J. Joosten for SlSOBIR1 and NbSOBIR1 constructs and TRV2::GUS, Caterina Brancato for assistance in tobacco transformation, and Franziska Hackbarth and Hermine Kienberger for excellent laboratory assistance at the BayBioMS. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG) to A.A.G. (SFB766, Gu 1034/3-1), the China Scholarship Council (CSC) to Y.Y. and the BMBF-funded de.NBI Cloud within the German Network for Bioinformatics Infrastructure (de.NBI). We acknowledge support from the Open Access Publication Fund of the University of Tübingen. Publisher Copyright: © 2023, The Author(s).
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