Arabidopsis latent virus 1, a comovirus widely spread in Arabidopsis thaliana collections
Verhoeven, Ava; Kloth, Karen J.; Kupczok, Anne; Oymans, Geert H.; Damen, Janna; Rijnsburger, Karin; Jiang, Zhang; Deelen, Cas; Sasidharan, Rashmi; Van zanten, Martijn; Van der vlugt, René A. A.
(2023) New Phytologist, volume 237, issue 4, pp. 1146 - 1153
(Article)
Abstract
Transcriptome studies of Illumina RNA-Seq datasets of different Arabidopsis thaliana natural accessions and T-DNA mutants revealed the presence of two virus-like RNA sequences which showed the typical two-segmented genome characteristics of a comovirus. This comovirus did not induce any visible symptoms in infected A. thaliana plants cultivated under standard laboratory conditions.
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Hence it was named Arabidopsis latent virus 1 (ArLV1). Virus infectivity in A. thaliana plants was confirmed by quantitative reverse transcription polymerase chain reaction, transmission electron microscopy and mechanical inoculation. Arabidopsis latent virus 1 can also mechanically infect Nicotiana benthamiana, causing distinct mosaic symptoms. A bioinformatics investigation of A. thaliana RNA-Seq repositories, including nearly 6500 Sequence Read Archives (SRAs) in the NCBI SRA database, revealed the presence of ArLV1 in 25% of all archived natural A. thaliana accessions and in 8.5% of all analyzed SRAs. Arabidopsis latent virus 1 could also be detected in A. thaliana plants collected from the wild. Arabidopsis latent virus 1 is highly seed-transmissible with up to 40% incidence on the progeny derived from infected A. thaliana plants. This has probably led to a worldwide distribution in the model plant A. thaliana with as yet unknown effects on plant performance in a substantial number of studies.
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Keywords: Arabidopsis latent virus 1 (ArLV1), Arabidopsis thaliana, RNA sequencing, comovirus, drought resilience, sequence read archives, Physiology, Plant Science
ISSN: 0028-646X
Publisher: Blackwell Publishing Ltd
Note: Funding Information: This research was supported by the Netherlands Organization for Scientific Research (NWO) (project no. 867.15.031 to AV, RS and MZ, and 016.VIDI.171.006 to RS), the China Scholarship Council (CSC) (project no. 201806170025 to ZJ), the Graduate School Experimental Plant Sciences (project V‐GENE to AK), and the NWO Technology Foundation Perspective Programme ‘Learning from Nature’ (STW10989) and the ZonMw programme ‘Enabling Technologies’ (435000017) to KJK. We wish to thank Emilyn Matsumura for critical reading of the manuscript, Rebecca Augsburger for technical assistance and Jan C. van Haarst for help with the analysis of the initial dataset containing the virus. We thank Cristian Carrasco‐López and Fernando García‐Arenal (CBGP, UPM‐INIA, Madrid) for helpful discussions and for sharing their unpublished data on the ArLV1 confirmation experiments performed on wild plants collected in Spain. Funding Information: This research was supported by the Netherlands Organization for Scientific Research (NWO) (project no. 867.15.031 to AV, RS and MZ, and 016.VIDI.171.006 to RS), the China Scholarship Council (CSC) (project no. 201806170025 to ZJ), the Graduate School Experimental Plant Sciences (project V-GENE to AK), and the NWO Technology Foundation Perspective Programme ‘Learning from Nature’ (STW10989) and the ZonMw programme ‘Enabling Technologies’ (435000017) to KJK. We wish to thank Emilyn Matsumura for critical reading of the manuscript, Rebecca Augsburger for technical assistance and Jan C. van Haarst for help with the analysis of the initial dataset containing the virus. We thank Cristian Carrasco-López and Fernando García-Arenal (CBGP, UPM-INIA, Madrid) for helpful discussions and for sharing their unpublished data on the ArLV1 confirmation experiments performed on wild plants collected in Spain. Publisher Copyright: © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
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