The transcription factor TCFL5 responds to A MYB to elaborate the male meiotic program in mice
Cecchini, Katharine; Biasini, Adriano; Yu, Tianxiong; Säflund, Martin; Mou, Haiwei; Arif, Amena; Eghbali, Atiyeh; Colpan, Cansu; Gainetdinov, Ildar; de Rooij, Dirk G; Weng, Zhiping; Zamore, Phillip; Ozata, Deniz M
(2023) Reproduction, volume 165, issue 2, pp. 183 - 196
(Article)
Abstract
In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis.
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This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.
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Keywords: TCFL5, spermatogenesis, meiosis, A-MYB, transcription factors network,, miR-34/449, Dna methylation, Motile ciliogenesis, Meiosis, Pirna production, Protein, Dynamics, Network, Clusters, Family, Mili, Taverne, Endocrinology, Obstetrics and Gynaecology, Cell Biology, Reproductive Medicine, Embryology
ISSN: 1470-1626
Publisher: BioScientifica Ltd.
Note: Funding Information: This work was supported in part by NIGMS grants R37 GM062862 and R35 GM136275 (PDZ), NICHHD grant P01 HD078253 (ZW and PDZ), and Swedish Research Council grant 2020-03818 (DMÖ), Carltryggersstiftelse CTS 21:1158 (DMÖ). The UMMS FACS Core is supported in part by NIH grant S10OD028576. Publisher Copyright: © 2023 Society for Reproduction and Fertility.
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