Insect eggs trigger systemic acquired resistance against a fungal and an oomycete pathogen
Alfonso, Esteban; Stahl, Elia; Glauser, Gaétan; Bellani, Etienne; Raaymakers, Tom M.; Van den Ackerveken, Guido; Zeier, Jürgen; Reymond, Philippe
(2021) New Phytologist, volume 232, issue 6, pp. 2491 - 2505
(Article)
Abstract
Plants are able to detect insect eggs deposited on leaves. In Arabidopsis, eggs of the butterfly species Pieris brassicae (common name large white) induce plant defenses and activate the salicylic acid (SA) pathway. We previously discovered that oviposition triggers a systemic acquired resistance (SAR) against the bacterial hemibiotroph pathogen Pseudomonas
... read more
syringae. Here, we show that insect eggs or treatment with egg extract (EE) induce SAR against the fungal necrotroph Botrytis cinerea BMM and the oomycete pathogen Hyaloperonospora arabidopsidis Noco2. This response is abolished in ics1, ald1 and fmo1, indicating that the SA pathway and the N-hydroxypipecolic acid (NHP) pathway are involved. Establishment of EE-induced SAR in distal leaves potentially involves tryptophan-derived metabolites, including camalexin. Indeed, SAR is abolished in the biosynthesis mutants cyp79B2 cyp79B3, cyp71a12 cyp71a13 and pad3-1, and camalexin is toxic to B. cinerea in vitro. This study reveals an interesting mechanism by which lepidopteran eggs interfere with plant–pathogen interactions.
show less
Download/Full Text
Keywords: Botrytis cinerea, herbivore interactions, indolic metabolism, insect eggs, Pieris brassicae, plant, systemic acquired resistance (SAR), Physiology, Plant Science
ISSN: 0028-646X
Publisher: Blackwell Publishing Ltd
Note: Funding Information: This research was supported by a grant (310030_200372) from the Swiss National Science Foundation to PR, by the Less is More grant (847.13.006) of the Netherlands Organization for Scientific Research to GVdA and TMR, and by a grant (ZE467/6–2) of the German Research Foundation (DFG) to JZ. We thank Blaise Tissot and Caroline Gouhier‐Darimont for maintenance of plants and insects, and Steve Lassueur for help with metabolite analyses. We thank N. Clay (Yale University, USA), E. Glawischnig (TUM Münich, Germany), I. Somssich (MPI Koeln, Germany) and Y. Zhang (University of British Columbia, CA) for sharing mutants. We thank the anonymous reviewers for useful comments on a previous version of the manuscript. Funding Information: This research was supported by a grant (310030_200372) from the Swiss National Science Foundation to PR, by the Less is More grant (847.13.006) of the Netherlands Organization for Scientific Research to GVdA and TMR, and by a grant (ZE467/6?2) of the German Research Foundation (DFG) to JZ. We thank Blaise Tissot and Caroline Gouhier-Darimont for maintenance of plants and insects, and Steve Lassueur for help with metabolite analyses. We thank N. Clay (Yale University, USA), E. Glawischnig (TUM M?nich, Germany), I. Somssich (MPI Koeln, Germany) and Y. Zhang (University of British Columbia, CA) for sharing mutants. We thank the anonymous reviewers for useful comments on a previous version of the manuscript. Publisher Copyright: © 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation
(Peer reviewed)
