A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles

DSpace/Manakin Repository

A human kidney and liver organoid-based multi-organ-on-a-chip model to study the therapeutic effects and biodistribution of mesenchymal stromal cell-derived extracellular vesicles
 
Nguyen, Vivian V T; Ye, Shicheng; Gkouzioti, Vasiliki; van Wolferen, Monique E; Yengej, Fjodor Yousef; Melkert, Dennis; Siti, Sofia; de Jong, Bart; Besseling, Paul J; Spee, Bart; van der Laan, Luc J W; Horland, Reyk; Verhaar, Marianne C; van Balkom, Bas W M
(2022) Journal of Extracellular Vesicles, volume 11, issue 11, pp. 1 - 20
(Article)
Abstract
Mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) show therapeutic potential in multiple disease models, including kidney injury. Clinical translation of sEVs requires further preclinical and regulatory developments, including elucidation of the biodistribution and mode of action (MoA). Biodistribution can be determined using labelled sEVs in animal models which come ... read more
Download/Full Text
Open Access version via Utrecht University Repository Publisher version
 
Keywords: Animals, Humans, Organoids, Tissue Distribution, Lab-On-A-Chip Devices, Extracellular Vesicles/metabolism, Mesenchymal Stem Cells, Liver, Kidney, 3Rs, EV-based therapeutics, micro-physiological models, renal injury
DOI: https://doi.org/10.1002/jev2.12280
ISSN: 2001-3078
Publisher: Co-Action Publishing
Note: Funding Information: The BIQMIMETICS collaboration project is co-funded by the PPP Allowance made available by Health-Holland, Top Sector Life Sciences & Health, to the Association of Collaborating Health Foundations (SGF) to stimulate public-private partnerships and by ZonMw (grant numbers LSHM20046-SGF and 114025102), the Dutch Kidney Foundation (grant number 19OP+007) and the Dutch Society for the Replacement of Animal Testing (Stichting Proefdiervrij). The REDUCE MORE! Project was funded by Health∼Holland TKI-LSH, grant number LSHM18045 (all to B.W.M. v.B.). This research was also funded by the Dutch Heart Foundation CVON2014-11 ‘RECONNECT’ and ‘RECONNEXT’ (to M.C.V.), the RECONNECT YTP ‘CHIPS’ grant and the Utrecht University 3Rs stimulus fund project “MOOI!” (to B.W.M.v.B.) grants; the EU H2020 research and innovation programme under Marie S. Curie Cofund RESCUE grant agreement No 801540 (to M.C.V.) and the Gravitation Program ‘Materials Driven Regeneration’, funded by the Netherlands Organization for Scientific Research (024.003.013) (to M.C.V.), China Scholarship Council (CSC201808310180) to (S.Y.), and Medical Delta program (Regenerative Medicine 4D) (to L.J.W.v.d.L.). We thank Dr Prins of the Gene and Cell Therapy facility of the UMC Utrecht for providing the MSC, Dr Verstegen for providing human liver organoids, and Dr Gremmels and Dr Maass for their help with data anaysis. The authors acknowledge the support of the partners of ‘Regenerative Medicine Crossing Borders’ (RegMed XB), Powered by Health∼Holland, Top Sector Life Sciences & Health. Funding Information: The BIQMIMETICS collaboration project is co‐funded by the PPP Allowance made available by Health‐Holland, Top Sector Life Sciences & Health, to the Association of Collaborating Health Foundations (SGF) to stimulate public‐private partnerships and by ZonMw (grant numbers LSHM20046‐SGF and 114025102), the Dutch Kidney Foundation (grant number 19OP+007) and the Dutch Society for the Replacement of Animal Testing (Stichting Proefdiervrij). The REDUCE MORE! Project was funded by Health∼Holland TKI‐LSH, grant number LSHM18045 (all to B.W.M. v.B.). This research was also funded by the Dutch Heart Foundation CVON2014‐11 ‘RECONNECT’ and ‘RECONNEXT’ (to M.C.V.), the RECONNECT YTP ‘CHIPS’ grant and the Utrecht University 3Rs stimulus fund project “MOOI!” (to B.W.M.v.B.) grants; the EU H2020 research and innovation programme under Marie S. Curie Cofund RESCUE grant agreement No 801540 (to M.C.V.) and the Gravitation Program ‘Materials Driven Regeneration’, funded by the Netherlands Organization for Scientific Research (024.003.013) (to M.C.V.), China Scholarship Council (CSC201808310180) to (S.Y.), and Medical Delta program (Regenerative Medicine 4D) (to L.J.W.v.d.L.). We thank Dr Prins of the Gene and Cell Therapy facility of the UMC Utrecht for providing the MSC, Dr Verstegen for providing human liver organoids, and Dr Gremmels and Dr Maass for their help with data anaysis. The authors acknowledge the support of the partners of ‘Regenerative Medicine Crossing Borders’ (RegMed XB), Powered by Health∼Holland, Top Sector Life Sciences & Health. Publisher Copyright: © 2022 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
(Peer reviewed)
 
See more statistics about this item