Potential Fungi Isolated From Anti-biodegradable Chinese Medicine Residue to Degrade Lignocellulose
Cheng, Min; Wijayawardene, Nalin N.; Promputtha, Itthayakorn; de Vries, Ronald P.; Lan, Yongzhe; Luo, Gang; Wang, Meizhu; Li, Qirui; Guo, Xinyao; Wang, Feng; Liu, Yanxia; Kang, Yingqian
(2022) Frontiers in Microbiology, volume 13
(Article)
Abstract
Traditional Chinese medicine is one of the ancient medicines which is popular in Asian countries, among which the residue produced by the use of anti-biodegradables is endless, and causes significant adverse impacts on the environment. However, the high acidity of anti-biodegradable residues and some special biological activities make it difficult
... read more
for microorganisms to survive, resulting in a very low degradation rate of lignocellulose in naturally stacked residues, which directly impedes the degradation of residues. We aimed to identify the fungal strains that efficiently biodegrade anti-biodegradable residue and see the possibility to improve the biodegradation of it and other agricultural wastes by co-cultivating these fungi. We isolated 302 fungal strains from anti-biodegradable residue to test hydrolysis ability. Finally, we found Coniochaeta sp., Fomitopsis sp., Nemania sp., Talaromyces sp., Phaeophlebiopsis sp. which inhabit the anti-biodegradable residues are capable of producing higher concentrations of extracellular enzymes. Synergistic fungal combinations (viz., Fomitopsis sp. + Phaeophlebiopsis sp.; Talaromyces sp. + Coniochaeta sp. + Fomitopsis sp.; Talaromyces sp. + Fomitopsis sp. + Piloderma sp. and Talaromyces sp. + Nemania sp. + Piloderma sp.) have better overall degradation effect on lignocellulose. Therefore, these fungi and their combinations have strong potential to be further developed for bioremediation and biological enzyme industrial production.
show less
Download/Full Text
Keywords: CAZymes, filamentous fungi, fungal diversity, hydrolysis, ITS, synergistic fungal combinations, Microbiology, Microbiology (medical)
ISSN: 1664-302X
Publisher: Frontiers Media S.A.
Note: Funding Information: This work was supported by grants from Guizhou Scientific Plan Project [(2019) 2873], the 111 Project (D20009), the National Natural Science Foundation of China (32060034), the Guizhou Scientifc Plan Project ([2020]4Y220), the International Science and Technology Cooperation Base of Guizhou Province [(2020) 4101], and Guizhou Provincial Academician Workstation of Microbiology and Health [(2020) 4004]. Funding Information: NW thanks the High-Level Talent Recruitment Plan of Yunnan Provinces (“Young Talents” Program and “High-End Foreign Experts” Program) and the Institute of Research and Development in Sri Lanka. IP, is grateful to Chiang Mai University for its partial support of this research work. Publisher Copyright: Copyright © 2022 Cheng, Wijayawardene, Promputtha, de Vries, Lan, Luo, Wang, Li, Guo, Wang, Liu and Kang.
(Peer reviewed)