GalR, GalX and AraR co-regulate d-galactose and l-arabinose utilization in Aspergillus nidulans
Meng, Jiali; Németh, Zoltán; Peng, Mao; Fekete, Erzsébet; Garrigues, Sandra; Lipzen, Anna; Ng, Vivian; Savage, Emily; Zhang, Yu; Grigoriev, Igor V.; Mäkelä, Miia R.; Karaffa, Levente; de Vries, Ronald P.
(2022) Microbial Biotechnology, volume 15, issue 6, pp. 1839 - 1851
(Article)
Abstract
Filamentous fungi produce a wide variety of enzymes in order to efficiently degrade plant cell wall polysaccharides. The production of these enzymes is controlled by transcriptional regulators, which also control the catabolic pathways that convert the released monosaccharides. Two transcriptional regulators, GalX and GalR, control d-galactose utilization in the model
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filamentous fungus Aspergillus nidulans, while the arabinanolytic regulator AraR regulates l-arabinose catabolism. d-Galactose and l-arabinose are commonly found together in polysaccharides, such as arabinogalactan, xylan and rhamnogalacturonan I. Therefore, the catabolic pathways that convert d-galactose and l-arabinose are often also likely to be active simultaneously. In this study, we investigated the interaction between GalX, GalR and AraR in d-galactose and l-arabinose catabolism. For this, we generated single, double and triple mutants of the three regulators, and analysed their growth and enzyme and gene expression profiles. Our results clearly demonstrated that GalX, GalR and AraR co-regulate d-galactose catabolism in A. nidulans. GalX has a prominent role on the regulation of genes of d-galactose oxido-reductive pathway, while AraR can compensate for the absence of GalR and/or GalX.
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Keywords: Beta-galactosidase, Catabolic pathway, D-xylose reductase, Dehydrogenase, Evolution, Identification, Missing link, Niger, Primary carbon metabolism, Transcriptional activator xlnr, Applied Microbiology and Biotechnology, Bioengineering, Biochemistry, Biotechnology
ISSN: 1751-7907
Publisher: John Wiley and Sons Ltd
Note: Funding Information: We thank Chinese Scholarship Council (CSC) for the financial support to J.M. (CSC student number: CSC201907720027). S.G. was supported by a grant of the Applied Science Division (TTW) of NWO and the Biotechnology and Safety Program of the Ministry of Infrastructure and Water Management 15807 to R.P.dV. The Academy of Finland grant number 308284 to M.R.M. is acknowledged. This research was supported by the Hungarian National Research, Development and Innovation Fund (grants NN128867 to L.K. and K138489 to E.F.). Publisher Copyright: © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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