Induced resistance to Fusarium wilt of banana caused by Tropical Race 4 in Cavendish cv Grand Naine bananas after challenging with avirulent Fusarium spp
García-Bastidas, Fernando A; Arango-Isaza, Rafael; Rodriguez-Cabal, Hector A; Seidl, Michael F; Cappadona, Giulio; Segura, Rafael; Salacinas, Maricar; Kema, Gert H J
(2022) PLoS One, volume 17, issue 9, pp. 1 - 19
(Article)
Abstract
In the last century, Fusarium wilt of banana (FWB) destroyed the banana cultivar Gros Michel. The Cavendish cultivars saved the global banana industry, and currently they dominate global production (~50%) and the export trade (~95%). However, a new strain called Tropical Race 4 (TR4) surfaced in the late 1960's, spread
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globally and greatly damages Cavendish plantations as well as manifold local varieties that are primarily grown by small holders. Presently, there is no commercially available replacement for Cavendish and hence control strategies must be developed and implemented to manage FWB. Here, we studied whether it is possible to induce resistance to TR4 by pre-inoculations with different Fusarium spp. Only pre-treatments with an avirulent Race 1 strain significantly reduced disease development of TR4 in a Cavendish genotype and this effect was stable at various nutritional and pH conditions. We then used transcriptome analysis to study the molecular basis of this response. Several genes involved in plant defence responses were up-regulated during the initial stages of individual infections with TR4 and Race 1, as well as in combined treatments. In addition, a number of genes in the ethylene and jasmonate response pathways as well as several gibberellin synthesis associated genes were induced. We observed upregulation of RGA2 like genes in all treatments. Hence, RGA2 could be a key factor involved in both R1 and TR4 resistance. The data support the hypothesis that activating resistance to Race 1 in Cavendish bananas affects TR4 development and provide a first insight of gene expression during the interaction between various Fusarium spp. and banana.
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Keywords: Ethylenes/metabolism, Fusarium, Gene Expression Regulation, Plant, Gibberellins/metabolism, Musa/genetics, Plant Diseases/genetics
ISSN: 1932-6203
Publisher: Public Library of Science
Note: Funding Information: This research is funded by the WURInterdisciplinary Research and Education Fund (INREF), comprising private and public partners, see www.fusariumwilt.org. Banana research at WUR is supported by the Dutch Dioraphte Foundation(https://www.dioraphte.nl) endowed chair in Tropical Phytopathology of GHJK at the WUR-Laboratory of Phytopathology. RA was funded by the Universidad Nacional de Colombia, Sede Medellín(https://medellin.unal.edu.co). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors would like to thank Dr. Eli Khayat, Rahan Meristem, Western Galilee, Israel, for providing plants evaluated in this program. We gratefully acknowledge our colleagues Marga van Gent for her support during the RNA isolation, Paul Keizer for the statistical analyses of the data and the Unifarm personnel for their support during trials and for greenhouse facility maintenance. We thank Harold Meijer for critically reading the manuscript. Publisher Copyright: © 2022 García-Bastidas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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