Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells
Korpershoek, Jasmijn V; Ruijter, Mylène de; Terhaard, Bastiaan F; Hagmeijer, Michella H; Saris, Daniël B F; Castilho, Miguel; Malda, Jos; Vonk, Lucienne A
(2021) International Journal of Molecular Sciences, volume 22, issue 20, pp. 1 - 16
(Article)
Abstract
Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using
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melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young's modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.
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Keywords: Biofabrication, Clinical translation, Collagen meniscus implant®, Melt electrowriting, Meniscectomy18, Meniscus, Meniscus injury, Tissue-engineering, Catalysis, Molecular Biology, Spectroscopy, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry, Inorganic Chemistry
ISSN: 1661-6596
Publisher: MDPI AG
Note: Funding Information: Funding: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program “Materials Driven Regeneration”, funded by the Netherlands Organization for Scientific Research Funding Information: This research received funding from the Dutch Arthritis Association (LLP-12 and LLP-22) from the European Union?s Horizon 2020 research and innovation programme under grant agreement No 814444 (MEFISTO). This research was financially supported by the Gravitation Program ?Materials Driven Regeneration?, funded by the Netherlands Organization for Scientific Research (024.003.013). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
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