Back to the Basics: Two Approaches for the Identification and Extraction of Lipid Droplets from Malassezia pachydermatis CBS1879 and Malassezia globosa CBS7966

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Back to the Basics: Two Approaches for the Identification and Extraction of Lipid Droplets from Malassezia pachydermatis CBS1879 and Malassezia globosa CBS7966
 
Mantilla, Maria Juliana; Cabrera Díaz, Catherine Eliana; Ariza-Aranguren, Gabriela; de Cock, Hans; Helms, J. Bernd; Restrepo, Silvia; Jiménez, Elizabeth; Celis Ramírez, Adriana Marcela
(2021) Current Protocols, volume 1, issue 5, pp. 1 - 16
(Article)
Abstract
Malassezia spp. are lipid-dependent yeasts that have been related to skin mycobiota and dermatological and systemic diseases. Study of lipid droplets (LDs) is relevant to elucidate the unknown role of these organelles in Malassezia and to gain a broader overview of lipid metabolism in Malassezia. Here, we standardized two protocols ... read more
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Keywords: cell wall rupture, fluorescence microscopy, lipid droplets, Malassezia globosa, Malassezia pachydermatis, Taverne, Biochemistry, Genetics and Molecular Biology(all), Pharmacology, Toxicology and Pharmaceutics(all), Neuroscience(all), Immunology and Microbiology(all), Health Informatics, Medical Laboratory Technology, Medicine(all)
DOI: https://doi.org/
ISSN: 2691-1299
Publisher: Wiley
Note: Funding Information: This work was supported by the Faculty of Sciences, Universidad de Los Andes, grant no. INV‐2018‐31‐1252, Project Semilla INV‐2018‐49‐1371, Project FAPA‐P17160322012, and the Vice‐Presidency of Research and Creation. We thank μ‐core at Universidad de Los Andes ( https://microcore.uniandes.edu.co/es/ ). Publisher Copyright: © 2021 Wiley Periodicals LLC
(Peer reviewed)
 
 
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