Immunophenotyping of the cutaneous cellular infiltrate after atopy patch testing in cats with atopic dermatitis

Abstract

Cats with spontaneously occurring atopic dermatitis have clinical and immunocytochemical characteristics compatible with these in humans with atopic dermatitis (AD). The atopy patch test (APT) has proven to be a valuable tool in elucidating the disease process in humans. Additionally, the APT is very specific and bypasses the problem of conflicting results due to differences in chronicity of lesions of AD patients. We adapted the APT for use in cats to explore the suitability of the APT as a tool to study the onset of allergic inflammation in cats with atopic dermatitis. APTwere performed in AD cats (n ¼ 6) and healthy cats (n ¼ 10). All cats were patch tested with two allergens in three different dilutions and a diluent control. The allergens for the APTwere selected from positive intradermal test and /or prick test results and consistedof:Dermatophagoidesfarinae,D.pteronyssinus,Tyrophagusputrescentiae,andagrasspollenmixture.APTwerereadafter 10, 24 and 48 h, and punch biopsies for immunohistochemical evaluation were collected at these time points. Macroscopically positive APT reactions were observed in three out of six cats at 24 and/or 48 h with allergen concentrations of 25 000 and 100 000 NU/ml. Reactions were not observed at negative control sites and neither in control animals. A significantly increased number of IL-4þ, CD4þ, CD3þ, MHC class IIþ and CD1aþ cells was found in one AD cat with positive APT reactions. Five out of six AD cats had significantly increased IL-4þ T cell numbers at 24 and/or 48 h. Our data indicate that in cats, macroscopically positive patch test reactions can be induced, which have a cellular infiltrate similar to that in lesional skin. We found a high specificity and a macroscopically positive APT reaction in half of the cats, which is similar to what is seen in humans. Hence, the APT in cats might be a useful tool in studying the immunopathogenesis of feline atopic dermatitis.