Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments
Masson, Glenn R; Burke, John E; Ahn, Natalie G; Anand, Ganesh S; Borchers, Christoph; Brier, Sébastien; Bou-Assaf, George M; Engen, John R; Englander, S Walter; Faber, Johan; Garlish, Rachel; Griffin, Patrick R; Gross, Michael L; Guttman, Miklos; Hamuro, Yoshitomo; Heck, Albert J R; Houde, Damian; Iacob, Roxana E; Jørgensen, Thomas J D; Kaltashov, Igor A; Klinman, Judith P; Konermann, Lars; Man, Petr; Mayne, Leland; Pascal, Bruce D; Reichmann, Dana; Skehel, Mark; Snijder, Joost; Strutzenberg, Timothy S; Underbakke, Eric S; Wagner, Cornelia; Wales, Thomas E; Walters, Benjamin T; Weis, David D; Wilson, Derek J; Wintrode, Patrick L; Zhang, Zhongqi; Zheng, Jie; Schriemer, David C; Rand, Kasper D
(2019) Nature Methods, volume 16, issue 7, pp. 595 - 602
(Article)
Abstract
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we
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provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.
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Keywords: Data Analysis, Deuterium Exchange Measurement/methods, Hydrogen-Ion Concentration, Mass Spectrometry/methods
ISSN: 1548-7091
Publisher: Nature Publishing Group
(Peer reviewed)
