Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures

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Long-term expansion and differentiation of adult murine epidermal stem cells in 3D organoid cultures
 
Boonekamp, Kim E.; Kretzschmar, Kai; Wiener, Dominique J.; Asra, Priyanca; Derakhshan, Sepideh; Puschhof, Jens; López-Iglesias, Carmen; Peters, Peter J.; Basak, Onur; Clevers, Hans
(2019) Proceedings of the National Academy of Sciences of the United States of America, volume 116, issue 29, pp. 14630 - 14638
(Article)
Abstract
Mammalian epidermal stem cells maintain homeostasis of the skin epidermis and contribute to its regeneration throughout adult life. While 2D mouse epidermal stem cell cultures have been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulating murine epidermal architecture has not been available. Here we describe an ... read more
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Keywords: Adult stem cells, Interfollicular epidermis, Mammalian epidermis, Organoids, General
DOI: https://doi.org/10.1073/pnas.1715272116
ISSN: 0027-8424
Publisher: National Academy of Sciences
Note: Funding Information: ACKNOWLEDGMENTS. We thank Joep Beumer for advice and critical reading of the manuscript, as well as contributions in the early stages of the project; Wilma Hoevenaar for imaging of the H2B-mNeonGreen–transduced organoids; Maaike van den Born and Johan H. van Es for help with animal procedures; Reinier van den Linden for and Stefan van der Elst help with flow cytometry; the Utrecht Sequencing Facility (USEQ) for sequencing; Anja van de Stolpe and Philips Research for their contributions to the project; the technical staff of the Microscopy CORE Lab of M4I Nanoscopy in Maastricht University and Willine van de Wetering for all their support in microscopy; and Anko de Graaff and the Hubrecht Imaging Center for help with imaging. H.C. gratefully acknowledges financial support from the Swiss National Science Foundation (Sinergia Grant CRSII3 160738-1) and the Netherlands Organization for Scientific Research (NWO-ZonMW, 114021012). This work is part of the Oncode Institute, which is partly financed by the Dutch Cancer Society and was funded by the gravitation program CancerGenomiCs.nl from the Netherlands Organization for Scientific Research (NWO). K.K. is a long-term fellow of the Human Frontier Science Program Organization (LT771/2015) and was the recipient of a VENI grant (NWO-ZonMW, 016.166.140). Funding Information: We thank Joep Beumer for advice and critical reading of the manuscript, as well as contributions in the early stages of the project; Wilma Hoevenaar for imaging of the H2B-mNeonGreen–transduced organoids; Maaike van den Born and Johan H. van Es for help with animal procedures; Reinier van den Linden for and Stefan van der Elst help with flow cytometry; the Utrecht Sequencing Facility (USEQ) for sequencing; Anja van de Stolpe and Philips Research for their contributions to the project; the technical staff of the Microscopy CORE Lab of M4I Nanoscopy in Maastricht University and Willine van de Wetering for all their support in microscopy; and Anko de Graaff and the Hubrecht Imaging Center for help with imaging. H.C. gratefully acknowledges financial support from the Swiss National Science Foundation (Sinergia Grant CRSII3 160738-1) and the Netherlands Organization for Scientific Research (NWO-ZonMW, 114021012). This work is part of the Oncode Institute, which is partly financed by the Dutch Cancer Society and was funded by the gravitation program CancerGenomiCs.nl from the Netherlands Organization for Scientific Research (NWO). K.K. is a long-term fellow of the Human Frontier Science Program Organization (LT771/2015) and was the recipient of a VENI grant (NWO-ZonMW, 016.166.140). Publisher Copyright: © 2019 National Academy of Sciences. All rights reserved.
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