CFTR - from function to phenotype

Abstract

In this thesis we have tried to unravel the complexity between CFTR genotype – CFTR function – CFTR phenotype a bit further. It is important to be able to measure CFTR function accurately, and to adequately determine disease severity and clinical response of an individual subject with CF. Especially at this moment as CFTR modulators are available to restore the disease in the basis. This thesis studies whether CFTR function measurements in intestinal organoids (‘mini guts’) are able to explain differences in clinical phenotype; also if the microbiome can be used as a new method to measure individual respiratory and intestinal outcome. Forskolin induced swelling (FIS) of intestinal organoids as new biomarker of CFTR function has shown to be a good predictor of respiratory and gastro-intestinal disease severity in CF and can differentiate between individuals with identical genotype but different phenotype. Microbiota data can complement current clinical outcome parameters to better define respiratory or gastro-intestinal phenotype as they differ between health and disease or differ between upper and lower airways and between patients or can detect response to therapy (antibiotics, CFTR modulators). These models will facilitate the development of future therapeutic and hopefully curative options for patients with CF.