A Comprehensive Human Gastric Cancer Organoid Biobank Captures Tumor Subtype Heterogeneity and Enables Therapeutic Screening
Yan, Helen H.N.; Siu, Hoi Cheong; Law, Simon; Ho, Siu Lun; Yue, Sarah S.K.; Tsui, Wai Yin; Chan, Dessy; Chan, April S.; Ma, Stephanie; Lam, Ka On; Bartfeld, Sina; Man, Alice H.Y.; Lee, Bernard C.H.; Chan, Annie S.Y.; Wong, Jason W.H.; Cheng, Priscilla S.W.; Chan, Anthony K.W.; Zhang, Jiangwen; Shi, Jue; Fan, Xiaodan; Kwong, Dora L.W.; Mak, Tak W.; Yuen, Siu Tsan; Clevers, Hans; Leung, Suet Yi
(2018) Cell Stem Cell, volume 23, issue 6, pp. 882 - 897.e11
(Article)
Abstract
Leung and colleagues established a biobank of patient-derived gastric cancer organoids that encompasses a diverse array of subtypes and maintained long-term similarity to the original tumors. They used the organoids to perform large-scale drug screening that identified potential target drugs and could guide patient drug selection.
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Keywords: ARHGAP fusions, biobank, drug screening, EBV genome, gastric cancer, heterogeneity, organoid culture, RHOA mutations, transcriptome sequencing, whole-exome sequencing, Molecular Medicine, Genetics, Cell Biology
ISSN: 1934-5909
Publisher: Cell Press
Note: Funding Information: We thank clinicians in the Hong Kong West Cluster, Hospital Authority for clinical care and sample coordination, and H. Wong, H.S. Hui, and H.C. Leung for technical support. We thank the Centre for Genomics Sciences, The University of Hong Kong for performing WES and RNA-seq experiments, and the Core Facility of the LKS Faculty of Medicine HKU for providing imaging and flow cytometry support. We also thank H.E. Francies and M.J. Garnett of Wellcome Trust Sanger Institute for advice on drug screening. This work was substantially supported by a theme-based research grant from the Research Grants Council of the Hong Kong Special Administrative Region, China (Project No. T12-710/16-R ), and partly by the Y.W. Kan Endowed Professorship established through a donation from the Croucher Foundation. Equipment support for drug screening was provided by the Jockey Club Centre for Clinical Innovation and Discovery. Publisher Copyright: © 2018 Elsevier Inc.
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