The HAUS Complex Is a Key Regulator of Non-centrosomal Microtubule Organization during Neuronal Development
Cunha-Ferreira, Inês; Chazeau, Anaël; Buijs, Robin R.; Stucchi, Riccardo; Will, Lena; Pan, Xingxiu; Adolfs, Youri; van der Meer, Christiaan; Wolthuis, Joanna C.; Kahn, Olga I.; Schätzle, Philipp; Altelaar, Maarten; Pasterkamp, R. Jeroen; Kapitein, Lukas C.; Hoogenraad, Casper C.
(2018) Cell Reports, volume 24, issue 4, pp. 791 - 800
(Article)
Abstract
Cunha-Ferreira et al. report that the HAUS/augmin complex regulates neuronal migration, polarization, and development. In neurons, the HAUS complex is distributed as discrete clusters regulating local microtubule nucleation. These findings shed light into how microtubules are generated in developing neurons after centrosome inactivation in early development.
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Keywords: actin, centrosome, development, microtubule, migration, neuron, nucleation, General Biochemistry,Genetics and Molecular Biology
ISSN: 2211-1247
Publisher: Cell Press
Note: Funding Information: We thank Gabriela Plucinska (Utrecht University) for critical reading of the manuscript. We thank Dr. Laurence Pelletier (Lunenfeld-Tanenbaum Research Institute) for sharing HAUS reagents and Dr. Ryota Uehara (Hokkaido University) and Prof. Gohta Goshima (Nagoya University) for the HAUS6 antibody. This work was supported by the following grants: Human Frontier Science Program fellowship LT000506/2013-L to I.C.-F.; the Netherlands Organization for Scientific Research (NWO), as part of the Graduate Programme project, grant 022.003.003 to R.R.B.; European Research Council (ERC) Consolidator Grant 617050 to C.C.H.; ERC Starting Grant 336291 to L.C.K.; Marie Curie fellowship 661401 to A.C.; NWO grant NWO-ALW-VICI 865.10.010 to C.C.H.; grant NWO-ALW-VICI 865.14.004 to R.J.P.; grant NWO-ALW-VIDI 864.12.008 to L.C.K.; NWO, as part of the National Roadmap Large-Scale Research Facilities of the Netherlands, Proteins@Work, grant 184.032.201 to M.A.; and VIDI grant 723.012.102 to M.A. Funding Information: We thank Gabriela Plucinska (Utrecht University) for critical reading of the manuscript. We thank Dr. Laurence Pelletier (Lunenfeld-Tanenbaum Research Institute) for sharing HAUS reagents and Dr. Ryota Uehara (Hokkaido University) and Prof. Gohta Goshima (Nagoya University) for the HAUS6 antibody. This work was supported by the following grants: Human Frontier Science Program fellowship LT000506/2013-L to I.C.-F.; the Netherlands Organization for Scientific Research (NWO), as part of the Graduate Programme project, grant 022.003.003 to R.R.B.; European Research Council (ERC) Consolidator Grant 617050 to C.C.H.; ERC Starting Grant 336291 to L.C.K.; Marie Curie fellowship 661401 to A.C.; NWO grant NWO-ALW-VICI 865.10.010 to C.C.H.; grant NWO-ALW-VICI 865.14.004 to R.J.P.; grant NWO-ALW-VIDI 864.12.008 to L.C.K.; NWO , as part of the National Roadmap Large-Scale Research Facilities of the Netherlands, Proteins@Work, grant 184.032.201 to M.A.; and VIDI grant 723.012.102 to M.A.
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