Engineering a 3d-bioprinted model of human heart valve disease using nanoindentation-based biomechanics

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Engineering a 3d-bioprinted model of human heart valve disease using nanoindentation-based biomechanics
 
van der Valk, Dewy C.; van der Ven, Casper F.T.; Blaser, Mark C.; Grolman, Joshua M.; Wu, Pin Jou; Fenton, Owen S.; Lee, Lang H.; Tibbitt, Mark W.; Andresen, Jason L.; Wen, Jennifer R.; Ha, Anna H.; Buffolo, Fabrizio; Mil, Alain van; Bouten, Carlijn V.C.; Body, Simon C.; Mooney, David J.; Sluijter, Joost P.G.; Aikawa, Masanori; Hjortnaes, Jesper; Langer, Robert; Aikawa, Elena
(2018) Nanomaterials, volume 8, issue 5
(Article)
Abstract
In calcific aortic valve disease (CAVD), microcalcifications originating from nanoscale calcifying vesicles disrupt the aortic valve (AV) leaflets, which consist of three (biomechanically) distinct layers: the fibrosa, spongiosa, and ventricularis. CAVD has no pharmacotherapy and lacks in vitro models as a result of complex valvular biomechanical features surrounding resident mechanosensitive ... read more
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Keywords: 3D printing, Aortic valve, Bioprinting, Calcific aortic valve disease, Calcification, Mechanobiology, Microdissection, Nanoindentation, General Materials Science, General Chemical Engineering
DOI: https://doi.org/10.3390/nano8050296
Publisher: Multidisciplinary Digital Publishing Institute
Note: Funding Information: This research was funded by the National Institutes of Health (NIH) R01 grants R01HL114805, R01HL136431 and R01HL109889 (E.A.); the NIH/National Institute of Dental and Craniofacial Research (NIDCR) R01 grant DE013033 (D.M.); the Netherlands CardioVascular Research Initiative (CVON: The Dutch Heart Foundation, Dutch Federation of University Medical Centers, the Netherlands Organization for Health Research and Development, and the Royal Netherlands Academy of Science) and Vrienden UMC Utrecht (C.V., J.S.); an unrestricted grant from CELLINK to Vrienden UMC Utrecht (C.V., J.S.); the Dutch Ministry of Education, Culture, and Science Gravitation Program grant 024.003.013 (C.V.C.B.); the Harvard Catalyst Advanced Microscopy Pilot grants (C.V., M.B., E.A.); and the NIH Ruth L. Kirschstein National Research Service Award F32HL122009 (M.W.T.). This work was conducted with support from Harvard Catalyst|The Harvard Clinical and Translational Science Center (National Center for Advancing Translational Sciences, National Institutes of Health Award UL1 TR001102) and financial contributions from Harvard University and its affiliated academic healthcare centers. This work was performed in part at Harvard University’s Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award No. 1541959. We thank the Harvard Center for Biological Imaging for infrastructure and support. Funding Information: Funding: This research was funded by the National Institutes of Health (NIH) R01 grants R01HL114805, R01HL136431 and R01HL109889 (E.A.); the NIH/National Institute of Dental and Craniofacial Research (NIDCR) R01 grant DE013033 (D.M.); the Netherlands CardioVascular Research Initiative (CVON: The Dutch Heart Foundation, Dutch Federation of University Medical Centers, the Netherlands Organization for Health Research and Development, and the Royal Netherlands Academy of Science) and Vrienden UMC Utrecht (C.V., J.S.); an unrestricted grant from CELLINK to Vrienden UMC Utrecht (C.V., J.S.); the Dutch Ministry of Education, Culture, and Science Gravitation Program grant 024.003.013 (C.V.C.B.); the Harvard Catalyst Advanced Microscopy Pilot grants (C.V., M.B., E.A.); and the NIH Ruth L. Kirschstein National Research Service Award F32HL122009 (M.W.T.). This work was conducted with support from Harvard Catalyst|The Harvard Clinical and Translational Science Center (National Center for Advancing Translational Sciences, National Institutes of Health Award UL1 TR001102) and financial contributions from Harvard University and its affiliated academic healthcare centers. This work was performed in part at Harvard University’s Center for Nanoscale Systems (CNS), a member of the National Nanotechnology Coordinated Infrastructure Network (NNCI), which is supported by the National Science Foundation under NSF award No. 1541959. We thank the Harvard Center for Biological Imaging for infrastructure and support. Publisher Copyright: © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
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