DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells
Harterink, Martin; da Silva, Marta Esteves; Will, Lena; Turan, Julia; Ibrahim, Adiljan; Lang, Alexander E; van Battum, Eljo Y; Pasterkamp, R Jeroen; Kapitein, Lukas C; Kudryashov, Dmitri; Barres, Ben A; Hoogenraad, Casper C; Zuchero, J Bradley
(2017) Nature Methods, volume 14, issue 5, pp. 479 - 482
(Article)
Abstract
The actin cytoskeleton is essential for many fundamental biological processes, but tools for directly manipulating actin dynamics are limited to cell-permeable drugs that preclude single-cell perturbations. Here we describe DeActs, genetically encoded actin-modifying polypeptides, which effectively induce actin disassembly in eukaryotic cells. We demonstrate that DeActs are universal tools for
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studying the actin cytoskeleton in single cells in culture, tissues, and multicellular organisms including various neurodevelopmental model systems.
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Keywords: ADP Ribose Transferases, Actin Cytoskeleton, Actins, Animals, Fibroblasts, Gelsolin, Green Fluorescent Proteins, HeLa Cells, Humans, Peptides, Rats, Recombinant Fusion Proteins, Transfection, Virulence Factors, Journal Article, Biotechnology, Molecular Biology, Biochemistry, Cell Biology, Journal Article
ISSN: 1548-7091
Publisher: Nature Publishing Group
Note: Publisher Copyright: © 2017 Nature America, Inc. part of Springer Nature. All rights reserved.
(Peer reviewed)