Asthmatic farm children show increased CD3+CD8low T-cells compared to non-asthmatic farm children
Twardziok, Monika; Schröder, Paul C; Krusche, Johanna; Casaca, Vera I; Illi, Sabina; Böck, Andreas; Loss, Georg J; Kabesch, Michael; Toncheva, Antoaneta A; Roduit, Caroline; Depner, Martin; Genuneit, Jon; Renz, Harald; Roponen, Marjut; Weber, Juliane; Braun-Fahrländer, Charlotte; Riedler, Josef; Lauener, Roger P.; Vuitton, Dominique Angèle; Dalphin, Jean-Charles; Pekkanen, Juha; von Mutius, Erika; Schaub, Bianca; Hyvärinen, Anne; Karvonen, Anne M; Kirjavainen, Pirkka V; Remes, Sami; Kaulek, Vincent; Dalphin, Marie-Laure; Ege, Markus Johannes; Pfefferle, Petra Ina; Doekes, Gert; PASTURE Study Group:
(2017) Clinical Immunology, volume 183, pp. 285 - 292
(Article)
Abstract
Several studies report an important role of CD8+ cytotoxic T-cells in atopy. Farm children show protection against atopy development, partly explained by CD4+ T-cell subtypes. Additional effects of CD8+ T-cells are unknown being investigated in this study within the PASTURE/EFRAIM birth cohort in PBMCs from farming and non-farming 6-year-old (N=76)
... read more
German children. CD3+CD8+CD25+ T-cells were analyzed by flow cytometry. Genotyping of 17q21 locus-SNPs associated with childhood asthma was performed. No differences in CD8+ T-cell subsets were seen between farmers and non-farmers regardless of asthma. Among farmchildren, asthmatics displayed increased CD3+CD8low(CD25+) T-cells compared to non-asthmatics. Asthmatic farm children exhibited a lower PI-induced stimulatory capacity of CD3+CD8low(CD25+) cells and a lower IFN-γ secretion than non-asthmatic farm children. Among farm children with GSDMB and ORMDL3 risk alleles, asthmatics displayed higher CD3+CD8low cells than non-asthmatics. Our data indicates a specific role of CD8low T-cells in asthmatic farm children.
show less
Download/Full Text
Keywords: CD8=+, CD8low, Childhood, farm-exposure, Taverne
ISSN: 1521-6616
Publisher: Academic Press Inc.
(Peer reviewed)
