DTI analysis methods: Region of interest analysis

Abstract

Region of interest (ROI) analysis is a widely used method for the analysis of DTI data. An anatomically defined region—either based on anatomical borders or a geometrical shape—is used to extract DTI measures for each subject, which can later be analyzed statistically. ROI analysis can be done either automatically by aligning all subjects to a template, or by manual delineation. In this chapter the basic principles of ROI analysis are discussed, as well as the appropriate use of ROI analysis and potential pitfalls. Finally some examples using real data are shown.