Evaluation of the sensitizing potential of food proteins using two mouse models
Smit, Joost; Zeeuw-Brouwer, Mary-Lène de; van Roest, Manon; de Jong, Govardus; van Bilsen, Jolanda
(2016) Toxicology Letters, volume 262, pp. 62 - 69
(Article)
Abstract
The current methodology to identify allergenic food proteins is effective in identifying those that are likely to cross-react with known allergens. However, most assays show false positive results for low/non-allergens. Therefore, an ex vivo/in vitro DC-T cell assay and an in vivo mouse model were used to distinguish known allergenic
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food proteins (Ara h 1, β-Lactoglobulin, Pan b 1, bovine serum albumin, whey protein isolate) from low/non allergenic food proteins (soy lipoxygenase, gelatin, beef tropomyosin, rubisco, Sola t 1). CD4+ T cells from protein/alum-immunized mice were incubated with corresponding protein-pulsed bone marrow-derived DC and analyzed for cytokine release. All known allergens induced Th2 responses in vitro, whereas soy lipoxygenase, gelatin or beef tropomyosin did not. Sola t 1 and rubisco induced a more generalized T cell response due to endotoxin contamination, indicating the endotoxin-sensitivity of the DC-T assay. To analyze responses in vivo, mice were orally sensitized on days 0 and 7. Known allergens induced IgE and mMCP-1 release upon oral challenge at day 16, whereas the low/non-allergens did not. Both the DC-T cell assay and the mouse model were able to distinguish 5 known allergens from 5 low/non-allergens and may be useful to identify novel allergenic food proteins.
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Keywords: Allergens, Animals, CD4-Positive T-Lymphocytes, Cell Line, Chemokine CCL2, Dietary Proteins, Disease Models, Animal, Food Hypersensitivity, Immunoglobulin E, Immunoglobulin G, Mice, Mice, Inbred C3H, T cells, Allergenic proteins, Dendritic cells, Food allergy, Mouse models, Sensitization
ISSN: 0378-4274
Publisher: Elsevier Ireland Ltd.
Note: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
(Peer reviewed)
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