Metabolic Engineering toward Sustainable Production of Nylon-6
Turk, Stefan C H J; Kloosterman, Wigard P.; Ninaber, Dennis K.; Kolen, Karin P A M; Knutova, Julia; Suir, Erwin; Schürmann, Martin; Raemakers-Franken, Petronella C.; Müller, Monika; De Wildeman, Stefaan M A; Raamsdonk, Leonie M.; Van Der Pol, Ruud; Wu, Liang; Temudo, Margarida F.; Van Der Hoeven, Rob A M; Akeroyd, Michiel; Van Der Stoel, Roland E.; Noorman, Henk J.; Bovenberg, Roel A L; Trefzer, Axel C.
(2016) ACS Synthetic Biology, volume 5, issue 1, pp. 65 - 73
(Article)
Abstract
Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways were identified for the fermentative production of 6-aminocaproic acid. Both pathways require yet unreported novel biocatalytic steps. We demonstrated proof
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of these bioconversions by in vitro enzyme assays with a set of selected candidate proteins expressed in Escherichia coli. One of the biosynthetic pathways starts with 2-oxoglutarate and contains bioconversions of the ketoacid elongation pathway known from methanogenic archaea. This pathway was selected for implementation in E. coli and yielded 6-aminocaproic acid at levels up to 160 mg/L in lab-scale batch fermentations. The total amount of 6-aminocaproic acid and related intermediates generated by this pathway exceeded 2 g/L in lab-scale fed-batch fermentations, indicating its potential for further optimization toward large-scale sustainable production of nylon-6.
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Keywords: 6-aminocaproic acid, adipate, caprolactam, metabolic engineering, nylon-6, α-ketopimelate, Biochemistry, Genetics and Molecular Biology (miscellaneous), Biomedical Engineering, Journal Article
ISSN: 2161-5063
Publisher: American Chemical Society
(Peer reviewed)