A specific mixture of non-digestible oligosaccharides enhances the tolerizing capacity of a partial whey hydrolysate in a mouse model for cow's milk allergy
Van Esch, Betty Cam; De Kivit, Sander; Hofman, Gerard A.; Nauta, Alma J.; Willemsen, Linette E. M.; Garssen, Johan; Knippels, Léon M. J.
(2011) Internal Medicine Journal, volume 41, issue Suppl. 4, pp. 1 - 12
(Abstract)
Abstract
Hypoallergenic infant formulas (HA) are considered a good alternative for infants at high risk for developing allergy if breastfeeding is not possible. Dietary intervention studies with HA combined with a specific mixture of non-digestible oligosaccharides, have been shown to reduce allergic symptoms in these children. However, the mechanisms by which
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these oligosaccharides exert their effect are yet to be explored. In this study, the contribution of this specific oligosaccharides mixture on the tolerizing capacity of a partial whey hydrolysate (WH) was investigated in a mice model of cow's milk allergy. Mice were sensitized orally with whey using cholera toxin as adjuvant. Prior to sensitization mice were pre-treated orally with partial WH, PBS, with or without supplementation with the specific oligosaccharide mixture containing short chain-galacto-, long chain-fructo- and acidic-oligosaccharides (9:1:1). After challenge, the acute allergic skin response, the mast cell mediator mMCP-1 and whey-specific antibodies were measured. The presence of Foxp3+regulatory T-cells and CD103+DC were determined in mesenteric lymph nodes. Oral pre-treatment of mice fed the partial WH induced tolerance as refl ected by a reduced acute allergic skin response and a suppressed mMCP-1 release without affecting whey-specific IgE levels. This effect coincided with increased CD103+DC and Foxp3+regulatory T-cell numbers. Interestingly, a combination of the partial WH and oligosaccharide diet completely abolished the acute allergic skin response and mMCP-1 release. In addition, a tendency towards decreased IgE levels and a further increase in intestinal CD103+DC numbers was observed. A specific mixture of non-digestible oligosaccharides enhanced the capacity of a partial WH to induce oral tolerance. This effect was associated with increased numbers of CD103+DC in the mesenteric lymph nodes, suggesting a role of these cells in the observed tolerance inducing capacity of this specific oligosaccharide mixture combined with partial WH.
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Keywords: oligosaccharide, immunoglobulin E, cholera toxin, adjuvant, antibody, society, mouse, whey, model, milk allergy, clinical immunology, allergy, skin, human, regulatory T lymphocyte, mesentery lymph node, artificial milk, infant, intervention study, child, risk, supplementation, mast cell, sensitization, cell count, diet
ISSN: 1444-0903
Publisher: Blackwell Publishing
Note: 22nd Australasian Society of Clinical Immunology and Allergy (ASCIA) Annual Scientific Meeting, Sydney Convention & Exhibition Centre, Darling Harbour, NSW, 7 - 9 September 2011