Abstract
Glucosylceramide (GlcCer) is synthesized at the cytosolic surface of the Golgi complex while enzymes acting in late steps of glycosphingolipid biosynthesis have their active centers in the Golgi lumen. However, the topology of the 'early' galactose-transferring enzymes is largely unknown. We used short-chain ceramides with either a 2-hydroxy fatty acid
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