Monolithic and Small Particle Column Materials for Application in Proteomics

Abstract

In this thesis the influence of the capillary liquid chromatography separation on the identification of protein digests is studied. In the first part the chromatographic parameters for silica monolithic columns are optimized to obtain a high throughput or a high separation performance. in the second part different column materials, monolithic and ultra-small particulate columns, are compared. In the last part trends and suggestions for future research are given.