Effect of diet composition on the protein synthetic pattern of the rat pancreas after a feeding period of five days

Abstract

Rats were fed for five days on a protein-rich and on a carbohydrate-rich diet, respectively. One half of the pancreas of these rats was incubated with [3H]-leucine and the other half with [14C]leucine and extracts from these pancreas halves were prepared. Mixtures of the differently labeled extracts were subjected to electrophoresis towards the anode as well as towards the cathode on a polyacrylamide gel containing urea at pH 8.5. Several secretory enzymes could be identified on the gels. Along the gels the 3H:14C ratio was determined in 1 mm slices. The results show that after five days of feeding a diet there is some adaptation to diet composition. Generally rather small changes in synthetic rate occur. Only one component, the cathodic chymotrypsinogen shows an important difference in synthetic rate under the two circumstances.