Genetic recombination in Escherichia coli IV. Isolation and characterization of recombinaion-deficient mutants of Escherichia coli K12

Abstract

19 independent recombination-deficient mutants were isolated. 7 carried mutations that mapped near or in the recB and recC genes between thyA and argA. 10 mutants carried mutations cotransducible with pheA and exhibited no complementation with recA in temporary zygotic diploids.

Two new genes controlling recombination have been identified. Strain PC 0297 carried a mutation designated recG162, and was UV- and X-ray-sensitive. It was located between pyrE and ilvA. Strain PC 1250 was only slightly UB-sensitive, was X-ray-resistant and carried the mutation designated recH166 which was located between pheA and cysQ.