Liquid chromatographic–mass spectrometric analysis of phospholipids. Chromatography, ionization and quantification

Abstract

This review deals with the LC-MS analysis of phospholipids. The advantages of including liquid chromatography in phospholipids are highlighted. Special attention is paid to the most-used ionization methods and the role of solvents in chromatography and ionization. Difficulties associated with different quantification strategies are discussed. This article is part of a Special Issue entitled Lipodomics and Imaging Mass Spectrometry.