Studies on protein synthesis by protoplasts of Saccharomyces carlsbergensis I. The effect of ribonuclease on protein synthesis

Abstract

Ribonuclease was found to inhibit the protein synthesis in the naked yeast protoplast for nearly 100%. Even small concentrations (5 μg/ml) were found inhibitory.

The cause of this inhibition can be attributed at least in part to a 90% inhibition of the respiration. Amino acid uptake was found to be inhibited for 75%, and glycolysis for 50%.